Q2K5P2 (FTHS_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	RHE_CH03078

Organism

Rhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]

Taxonomic identifier

347834 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium › Rhizobium etli

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000293052
Regions
	Nucleotide binding	68 – 75	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2K5P2 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 77EE4A7AB92E53A6	FASTA	559	59,740
	10 20 30 40 50 60 MPSIKSDIEI ARAATKRPIF EIGAKLGIAA EQLVPYGHDK AKVSAEFIAA QAGKKDGKLI 70 80 90 100 110 120 LVTAINPTPA GEGKTTTTVG LGDGLNRIGK KAIVCIREAS LGPCFGVKGG AAGGGYAQVV 130 140 150 160 170 180 PMEDINLHFT GDFHAITSAH NLLAAIIDNH IYWGNEENID IRRITWRRVM DMNDRALRSM 190 200 210 220 230 240 ISSLGGVANG FPRQGGFDIT VASEVMAILC LATDLKDLER RLGDIIIGYR FDKTPVHARD 250 260 270 280 290 300 LKADGAMAVL LKDAMQPNLV QTLESNPAFV HGGPFANIAH GCNSVTATKT ALKLGEYVVT 310 320 330 340 350 360 EAGFGADLGA EKFFDIKCRK AGLRPDAAVI VATVRALKMN GGVKKEDLGT EDVAALKKGC 370 380 390 400 410 420 ANLGRHVANV RRFGVPVVVA INHFVSDTDA EIAAVKEFVS RLGAEAILCQ HWAKGSAGIE 430 440 450 460 470 480 ELAHKVVELA ESGQAKFQPL YGDDISLFEK IEIIASKIYH AGEVTADKAV RDQLQSWEEQ 490 500 510 520 530 540 GYGKLPVCMA KTQYSFSTDP NLRGAPEGHI VSVREVRLSA GAGFVVAITG EIMTMPGLPK 550 SPSAERIFLN DQGYIEGLF

References

[1]

"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons." Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G. Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFN 42 / ATCC 51251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000133 Genomic DNA. Translation: ABC91844.1.
RefSeq	YP_470571.1. NC_007761.1.
3D structure databases
ProteinModelPortal	Q2K5P2.
SMR	Q2K5P2. Positions 7-557.
ModBase	Search...
Protein-protein interaction databases
STRING	347834.RHE_CH03078.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC91844; ABC91844; RHE_CH03078.
GeneID	3894039.
KEGG	ret:RHE_CH03078.
PATRIC	23087793. VBIRhiEtl108884_3547.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	EIMAVLC.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	RETL347834:GJJ0-3092-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_RHIEC

Accession

Primary (citable) accession number: Q2K5P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 7, 2006
Last modified:	May 29, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program