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Q2K487 (GLGB1_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB 1

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1
Alpha-(1->4)-glucan branching enzyme 1
Glycogen branching enzyme 1
Short name=BE 1
Gene names
Name:glgB1
Ordered Locus Names:RHE_CH03594
OrganismRhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]
Taxonomic identifier347834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length735 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7357351,4-alpha-glucan branching enzyme GlgB 1 HAMAP-Rule MF_00685
PRO_0000260682

Sites

Active site4181Nucleophile By similarity
Active site4711Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2K487 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: D9558CC7C5C8D71B

FASTA73583,272
        10         20         30         40         50         60 
MKTPKTLPEV TLSWEISADE ITAILAGSHS NPFAVLGVHQ GGDAFVARCF IPGAEEVTAM 

        70         80         90        100        110        120 
TLDGGFIGEL KQLHADGVFA GPVSLAKLQP VRYRARRGDA EWAVTDPYSF GPVLGPMDDY 

       130        140        150        160        170        180 
FAREGSHLRL FDKMGAHLIK HEGAQGIHFA VWAPNAQRVS VVGDFNGWDG RRHVMRFRAD 

       190        200        210        220        230        240 
SGIWEIFAPD VPLGVAYKFE IRGHDGVLLP LKADPFARRS ELRPKTASIA AAELEQVWED 

       250        260        270        280        290        300 
EAHLKHWRET DKRRQPISIY EVHAGSWQRR QDGTMLSWDE LASSLIPYCV DMGFTHIEFL 

       310        320        330        340        350        360 
PITEHPYDPS WGYQTTGLYA PTARFGEPEG FARFVNGCHK VGIGVILDWV PAHFPTDEHG 

       370        380        390        400        410        420 
LGWFDGTALY EHEDPRKGFH PDWNTAIYNF GRTEVVSYLV NNALYWAEKF HLDGLRVDAV 

       430        440        450        460        470        480 
ASMLYLDYSR KHGEWIPNEY GGNENLEAVR FLQDLNIRIY GQHSNVMTIA EESTSWPKVS 

       490        500        510        520        530        540 
QPVHEGGLGF GFKWNMGFMH DTLSYMKRDP VHRKHHHNEL TFGLLYAYSE NFVLPLSHDE 

       550        560        570        580        590        600 
VVHGKGSLIA KMPGDDWQKF ANLRAYYAYM WGYPGKKLLF MGQEFAQWSE WSEAKSLDWN 

       610        620        630        640        650        660 
LLQYRMHEGM RRLVRDLNFT YRSKPALHER DCEGEGFEWL VADDHQNSVF AWLRKAPGQK 

       670        680        690        700        710        720 
PVAVITNFTP VYRENYTIRL PSAGRWREIL NTDADIYGGS GKGNGGRVQA VDAGGDITCS 

       730 
ITLPPLATIM LEPEN 

« Hide

References

[1]"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons."
Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G.
Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CFN 42 / ATCC 51251.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000133 Genomic DNA. Translation: ABC92349.1.
RefSeqYP_471076.1. NC_007761.1.

3D structure databases

ProteinModelPortalQ2K487.
SMRQ2K487. Positions 126-735.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING347834.RHE_CH03594.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC92349; ABC92349; RHE_CH03594.
GeneID3894246.
KEGGret:RHE_CH03594.
PATRIC23088877. VBIRhiEtl108884_4080.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAWIERINT.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycRETL347834:GJJ0-3609-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB1_RHIEC
AccessionPrimary (citable) accession number: Q2K487
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: March 7, 2006
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries