Q2K487 (GLGB1_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB 1
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1
Alpha-(1->4)-glucan branching enzyme 1
Glycogen branching enzyme 1
Short name=BE 1

Gene names

Name:	glgB1
Ordered Locus Names:	RHE_CH03594

Organism

Rhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]

Taxonomic identifier

347834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	1,4-alpha-glucan branching enzyme GlgB 1 HAMAP MF_00685		PRO_0000260682
Sites
	Active site	418	1	Nucleophile By similarity
	Active site	471	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2K487 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: D9558CC7C5C8D71B	FASTA	735	83,272
	10 20 30 40 50 60 MKTPKTLPEV TLSWEISADE ITAILAGSHS NPFAVLGVHQ GGDAFVARCF IPGAEEVTAM 70 80 90 100 110 120 TLDGGFIGEL KQLHADGVFA GPVSLAKLQP VRYRARRGDA EWAVTDPYSF GPVLGPMDDY 130 140 150 160 170 180 FAREGSHLRL FDKMGAHLIK HEGAQGIHFA VWAPNAQRVS VVGDFNGWDG RRHVMRFRAD 190 200 210 220 230 240 SGIWEIFAPD VPLGVAYKFE IRGHDGVLLP LKADPFARRS ELRPKTASIA AAELEQVWED 250 260 270 280 290 300 EAHLKHWRET DKRRQPISIY EVHAGSWQRR QDGTMLSWDE LASSLIPYCV DMGFTHIEFL 310 320 330 340 350 360 PITEHPYDPS WGYQTTGLYA PTARFGEPEG FARFVNGCHK VGIGVILDWV PAHFPTDEHG 370 380 390 400 410 420 LGWFDGTALY EHEDPRKGFH PDWNTAIYNF GRTEVVSYLV NNALYWAEKF HLDGLRVDAV 430 440 450 460 470 480 ASMLYLDYSR KHGEWIPNEY GGNENLEAVR FLQDLNIRIY GQHSNVMTIA EESTSWPKVS 490 500 510 520 530 540 QPVHEGGLGF GFKWNMGFMH DTLSYMKRDP VHRKHHHNEL TFGLLYAYSE NFVLPLSHDE 550 560 570 580 590 600 VVHGKGSLIA KMPGDDWQKF ANLRAYYAYM WGYPGKKLLF MGQEFAQWSE WSEAKSLDWN 610 620 630 640 650 660 LLQYRMHEGM RRLVRDLNFT YRSKPALHER DCEGEGFEWL VADDHQNSVF AWLRKAPGQK 670 680 690 700 710 720 PVAVITNFTP VYRENYTIRL PSAGRWREIL NTDADIYGGS GKGNGGRVQA VDAGGDITCS 730 ITLPPLATIM LEPEN

References

[1]

"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons." Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G. Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed: 16505379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFN 42 / ATCC 51251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000133 Genomic DNA. Translation: ABC92349.1.
RefSeq	YP_471076.1. NC_007761.1.
3D structure databases
ProteinModelPortal	Q2K487.
SMR	Q2K487. Positions 126-735.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2K487.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3894246.
GenomeReviews	Gene locus RHE_CH03594 in contig CP000133_GR.
KEGG	ret:RHE_CH03594.
PATRIC	23088877. VBIRhiEtl108884_4080.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	WEIFAPD.
PhylomeDB	Q2K487.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB1_RHIEC

Accession

Primary (citable) accession number: Q2K487

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program