Q2K3M6 (GLO2_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	RHE_CH03812

Organism

Rhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]

Taxonomic identifier

347834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309685
Sites
	Metal binding	57	1	Zinc 1 By similarity
	Metal binding	59	1	Zinc 1 By similarity
	Metal binding	61	1	Zinc 2 By similarity
	Metal binding	62	1	Zinc 2 By similarity
	Metal binding	115	1	Zinc 1 By similarity
	Metal binding	134	1	Zinc 1 By similarity
	Metal binding	134	1	Zinc 2 By similarity
	Metal binding	172	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2K3M6 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 0F352C059D30C516	FASTA	256	28,559
	10 20 30 40 50 60 MKPLELDVFL CRTDNFGVLV HDPETGFTAA IDAPEEAPIL EAATRRGWKI THIFTTHHHT 70 80 90 100 110 120 DHVAANLALK EQFGCEIIGP INEAIAIPGL DRTMADGDSF LFGDHTVKVI ETPGHTAGHI 130 140 150 160 170 180 CYHFVDDKLL FAADTLFALG CGRLFERPAA DMWHSLQKLA VLPDETAVYF GHEYTLSNAR 190 200 210 220 230 240 FALTVDPDNE RLKSRAAEIE ALRAEGKFTV PTTLGLEKET NPFLRAADPA IRRHLIMEGK 250 TNEEVFAEIR KRKDNF

References

[1]

"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons." Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G. Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed: 16505379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFN 42 / ATCC 51251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000133 Genomic DNA. Translation: ABC92560.1.
RefSeq	YP_471287.1. NC_007761.1.
3D structure databases
ProteinModelPortal	Q2K3M6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2K3M6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3894432.
GenomeReviews	Gene locus RHE_CH03812 in contig CP000133_GR.
KEGG	ret:RHE_CH03812.
PATRIC	23089341. VBIRhiEtl108884_4306.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	FLCRTDN.
PhylomeDB	Q2K3M6.
ProtClustDB	CLSK800959.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_RHIEC

Accession

Primary (citable) accession number: Q2K3M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program