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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-4134-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:RHE_CH04116
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000001936 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Leucine--tRNA ligasePRO_1000009407Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi347834.RHE_CH04116.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi632 – 6365"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2K2S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATERYNPRD AEPRWQQKWN EDKVFETDNS DPREKYYVLE MFPYPSGRIH
60 70 80 90 100
MGHVRNYAMG DVVARYKRAR GYNVLHPMGW DAFGMPAENA AMERGVHPAS
110 120 130 140 150
WTYQNIASMK AQLKAMGLSL DWSREFATCD VDYYQHQQHL FLDFLEKGLV
160 170 180 190 200
YRKQSKVNWD PVDNTVLANE QVIDGRGWRS GALVEQRELT QWFFKITDFS
210 220 230 240 250
QELLDALDTL DQWPEKVRLM QKNWIGRSEG LTVRWEIVPE TAPAGETEIT
260 270 280 290 300
VYTTRPDTLF GASFLAIAAD HPLAKDAAAK NVEIEAFCEE CRRAGTSLAA
310 320 330 340 350
LETAEKKGLD TGIRVRHPLD PSWELPVYIA NFVLMDYGTG AIFGCPSGDQ
360 370 380 390 400
RDLDFARKYG LPVVPVVMPT DGDAASFSVG DTAYDGEGVM INSRFLDGKT
410 420 430 440 450
TEEAFNIVAD RLSAASLGNA PQGQRMINFR LRDWGISRQR YWGCPIPVIH
460 470 480 490 500
CDACGVVPVP KKDLPVKLPE DVTFDQPGNP LDRHPTWRHV ACPNCGKDAR
510 520 530 540 550
RETDTMDTFV DSSWYFTRFT APWEAKPTDP EAANRWLPVD QYIGGIEHAI
560 570 580 590 600
LHLLYSRFFT RAMRETGHVA ATEPFKGLFT QGMVVHETYS RGAGASREWV
610 620 630 640 650
APADIRIEEI DGKRRAFLLA SDEEVAIGSI EKMSKSKKNV VDPDDIIASY
660 670 680 690 700
GADTARFFVL SDSPPERDVI WSEAGVEGAH RFTQRLWRLI SEAADCLSAV
710 720 730 740 750
APAPASEGEA LTVSQAAHKT LKAVQNDYDK LWFNKAVARI YELVNALAAP
760 770 780 790 800
LTRVAAGEGD IAYRAAVRDA AEILIQLVAP MTPHLAEECW AALGNAGLLA
810 820 830 840 850
RTDWPRYDET LVMENDVVLP VQINGKKRAE LTISRDADQN AVTNAVLDLD
860 870
AVKNALNGQA PKKIIVVPQR IVNIVV
Length:876
Mass (Da):98,000
Last modified:March 7, 2006 - v1
Checksum:i79E5B43E3AEFEBD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000133 Genomic DNA. Translation: ABC92859.1.
RefSeqiYP_471586.1. NC_007761.1.

Genome annotation databases

EnsemblBacteriaiABC92859; ABC92859; RHE_CH04116.
KEGGiret:RHE_CH04116.
PATRICi23089986. VBIRhiEtl108884_4625.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000133 Genomic DNA. Translation: ABC92859.1.
RefSeqiYP_471586.1. NC_007761.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi347834.RHE_CH04116.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC92859; ABC92859; RHE_CH04116.
KEGGiret:RHE_CH04116.
PATRICi23089986. VBIRhiEtl108884_4625.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-4134-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFN 42 / ATCC 51251.

Entry informationi

Entry nameiSYL_RHIEC
AccessioniPrimary (citable) accession number: Q2K2S7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: April 1, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.