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Q2K0X8 (THIM_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:RHE_PE00337
Encoded onPlasmid p42e
OrganismRhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]
Taxonomic identifier347834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length258 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 258258Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000383889

Sites

Binding site371Substrate; via amide nitrogen By similarity
Binding site1121ATP By similarity
Binding site1581ATP By similarity
Binding site1851Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2K0X8 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 1E56309883AB05F9

FASTA25826,338
        10         20         30         40         50         60 
MLDAMREKPP LVHCITNYVA MNIAANVLLA SGASPAMVHA PEEAGEFAGI ASALTVNIGT 

        70         80         90        100        110        120 
LSTQWIDGMQ AAAKAAASAG KPWVLDPVAH YATTFRRQAV ADLLALKPTI IRGNASEIIA 

       130        140        150        160        170        180 
LAGGESRGQG VDSRDPVEQA EDSARRLAER QQAIVAVTGA VDFVTDGERA VRIKGGSVLM 

       190        200        210        220        230        240 
PEVTALGCSL TCLIGAFAAT APEDLFGATV AALATFAVAG EDAALGAAGP GSFAWRFLDA 

       250 
LAALDGEALD ARARVSLA 

« Hide

References

[1]"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons."
Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G.
Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CFN 42 / ATCC 51251.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000137 Genomic DNA. Translation: ABC93772.1.
RefSeqYP_472499.1. NC_007765.1.

3D structure databases

ProteinModelPortalQ2K0X8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING347834.RHE_PE00337.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC93772; ABC93772; RHE_PE00337.
GeneID3895735.
KEGGret:RHE_PE00337.
PATRIC23091948. VBIRhiEtl108884_5603.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMAMANEAPE.
OrthoDBEOG628F8M.

Enzyme and pathway databases

BioCycRETL347834:GJJ0-5054-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_RHIEC
AccessionPrimary (citable) accession number: Q2K0X8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 7, 2006
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways