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Protein

Catalase-peroxidase

Gene

katG

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Important for stationary phase survival.UniRule annotation1 Publication

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei93 – 931Transition state stabilizerUniRule annotation
Active sitei97 – 971Proton acceptorUniRule annotation
Metal bindingi259 – 2591Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-4148-MONOMER.

Protein family/group databases

PeroxiBasei2371. RetCP01_CFN42.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:RHE_PF00004
Encoded oniPlasmid p42f1 Publication
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000001936 Componenti: Plasmid p42f

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 728728Catalase-peroxidasePRO_0000354882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki96 ↔ 218Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-244)UniRule annotation
Cross-linki218 ↔ 244Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Proteomic databases

PRIDEiQ2JZT8.

Expressioni

Inductioni

Expression increases from exponential to early- and late-stationary phase. Induced by H2O2.1 Publication

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2JZT8.
SMRiQ2JZT8. Positions 21-727.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2JZT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNPTDTAGK CPVAHGNKPR GPSNRDWWPN QLNVQILHHN SGRADPLGKD
60 70 80 90 100
FDYAEEFKKL DLDALKKDLH ALMTDSQDWW PADFGHYGGL FIRMAWHSAG
110 120 130 140 150
TYRITDGRGG AGQGQQRFAP LNSWPDNANL DKARRLLWPI KQKYGNRISW
160 170 180 190 200
ADLLILTGNV ALESMGFKTF GFAGGRADVW EPEELYWGPE GTWLGDERYS
210 220 230 240 250
GERQLAEPLG AVQMGLIYVN PEGPNGNPDP VAAARDIRET FARMAMNDEE
260 270 280 290 300
TVALIAGGHT FGKTHGAGDP SFIGAEPEGG AIEDQGLGWK SSFGTGVGKD
310 320 330 340 350
AITAGLEVTW SQTPTKWSNY FFENLFAYEW ELTKSPAGAH QWRAKNAEAS
360 370 380 390 400
IPDAYEPGKK HVPTMLTTDL SLRFDPIYEK ISRRFLENPD QFADAFARAW
410 420 430 440 450
FKLTHRDMGP KVRYLGPEVP AEDLIWQDVI PAVDHPLVDD KDIAELKAKV
460 470 480 490 500
LATGLTVQEL VSTAWASAST FRGSDKRGGA NGARIRLAPQ KDWEANQPAQ
510 520 530 540 550
LAKVLGVLEG IQKDFNAAQT GAKKISLADL IVLAGAAGVE KAAAAGGNAV
560 570 580 590 600
SVPLTPGRMD ASEAQTDAHS FAPLEPRIDG FRNYVNGKRL QFMKPEEALV
610 620 630 640 650
DRAQLLTLTG PEMTVLVGGL RVLKAGNPEH GVFTSRPETL TNDFFVNLLD
660 670 680 690 700
VATQWVPATG KEGVYEGRDR KTGAAKWTGT RVDLIFGSHS QLRAFAEVYG
710 720
QADAKQKFVK DFVAAWNKVM NADRFDLV
Length:728
Mass (Da):79,871
Last modified:March 7, 2006 - v1
Checksum:iA4329443428299BE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 6813EFKKL…ALKKD → DIQEARSRRAEKG in AAL93241 (PubMed:12724378).CuratedAdd
BLAST
Sequence conflicti113 – 1131Q → H in AAL93241 (PubMed:12724378).Curated
Sequence conflicti116 – 1161Q → H in AAL93241 (PubMed:12724378).Curated
Sequence conflicti170 – 1701F → L in AAL93241 (PubMed:12724378).Curated
Sequence conflicti176 – 1761R → C in AAL93241 (PubMed:12724378).Curated
Sequence conflicti204 – 2041Q → H in AAL93241 (PubMed:12724378).Curated
Sequence conflicti207 – 2071E → N in AAL93241 (PubMed:12724378).Curated
Sequence conflicti241 – 2411F → L in AAL93241 (PubMed:12724378).Curated
Sequence conflicti415 – 4151L → F in AAL93241 (PubMed:12724378).Curated
Sequence conflicti419 – 4191V → L in AAL93241 (PubMed:12724378).Curated
Sequence conflicti437 – 4371L → F in AAL93241 (PubMed:12724378).Curated
Sequence conflicti540 – 5401E → K in AAL93241 (PubMed:12724378).Curated
Sequence conflicti598 – 5981A → M in AAL93241 (PubMed:12724378).Curated
Sequence conflicti652 – 6532AT → GD in AAL93241 (PubMed:12724378).Curated
Sequence conflicti659 – 66911TGKEGVYEGRD → PERKGLYRPR in AAL93241 (PubMed:12724378).CuratedAdd
BLAST
Sequence conflicti728 – 7281V → R in AAL93241 (PubMed:12724378).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486647 Genomic DNA. Translation: AAL93241.1.
CP000138 Genomic DNA. Translation: ABC93898.1.
RefSeqiWP_011428316.1. NC_007766.1.

Genome annotation databases

EnsemblBacteriaiABC93898; ABC93898; RHE_PF00004.
GeneIDi24303355.
KEGGiret:RHE_PF00004.
PATRICi23092217. VBIRhiEtl108884_5737.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486647 Genomic DNA. Translation: AAL93241.1.
CP000138 Genomic DNA. Translation: ABC93898.1.
RefSeqiWP_011428316.1. NC_007766.1.

3D structure databases

ProteinModelPortaliQ2JZT8.
SMRiQ2JZT8. Positions 21-727.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

PeroxiBasei2371. RetCP01_CFN42.

Proteomic databases

PRIDEiQ2JZT8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC93898; ABC93898; RHE_PF00004.
GeneIDi24303355.
KEGGiret:RHE_PF00004.
PATRICi23092217. VBIRhiEtl108884_5737.

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-4148-MONOMER.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKATG_RHIEC
AccessioniPrimary (citable) accession number: Q2JZT8
Secondary accession number(s): Q8RMZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: March 7, 2006
Last modified: September 7, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.