Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q2JZ21 (GLGB2_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB 2

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-(1->4)-glucan branching enzyme 2
Glycogen branching enzyme 2
Short name=BE 2
Gene names
Name:glgB2
Ordered Locus Names:RHE_PF00275
Encoded onPlasmid p42f
OrganismRhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]
Taxonomic identifier347834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length732 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7327321,4-alpha-glucan branching enzyme GlgB 2 HAMAP-Rule MF_00685
PRO_0000260683

Sites

Active site4131Nucleophile By similarity
Active site4661Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2JZ21 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 23B9D25D7E41BA0E

FASTA73281,931
        10         20         30         40         50         60 
MNVERSEFLA GVGHDALWAL IEGRHGDPFS ILGPHESGGM TIVRVYLPGA EGVDLIEAAS 

        70         80         90        100        110        120 
GRVVTPFSIA HPSGLFAAAM GSRMHYRLRI TWPDGEQITE DPYSFGLLLG ELDLHLISEG 

       130        140        150        160        170        180 
THYSLSRTLG AVEMAIDDVA GVRFAVWAPN ARRVSVVGDF NAWDGRRNPM RLRQSAGVWE 

       190        200        210        220        230        240 
LFMPRLAPGE RYKFEIIDPH GNCLPQKADP VARASEAAPS TASIVASSTR FRWTDDNWMK 

       250        260        270        280        290        300 
GQSRQQRLEG PISVYEVHAG SWLRENGGRS LDWVELSQRL VPYVREMGFT HIELLPIMEH 

       310        320        330        340        350        360 
PFGGSWGYQP LGLFAPTGRY GTPEDLAYFI DRCHGAGIGV ILDWVPAHFP TDVWGLARFD 

       370        380        390        400        410        420 
GTALYEHEDP REGFHRDWNT LIYNLGRNEV KGFLIASALE WLERYHIDGL RVDAVASMLY 

       430        440        450        460        470        480 
RDYSRNEGEW IPNRYGGREN LEAVEFFKHL NSIVHERCPH AMMIAEESTA WPGVTKPPEE 

       490        500        510        520        530        540 
GGLGFDMKWN MGWMHDSLSY IEKDPVYRSY HHGTMTFGMI YAYSERFILP ISHDEVVYGK 

       550        560        570        580        590        600 
GSLLGKMPGD EWQKFANLRS YLAFMWGHPG KKLIFMGGEI AQPSEWNHDA SIAWDVLDQP 

       610        620        630        640        650        660 
AHAGLQRLVK DLNGFYKDEA ALQFGDFHSE GFDWAAADDA VNSVLGMLRY APDRSSSVLV 

       670        680        690        700        710        720 
VSNFTPVPRY GYRIGVPQDG VWIEKVTTDA REYGGSGLVN GAVSSESVPA HGRPHSLWLT 

       730 
LPPLATVLLK SP 

« Hide

References

[1]"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons."
Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G.
Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CFN 42 / ATCC 51251.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000138 Genomic DNA. Translation: ABC94165.1.
RefSeqYP_472892.1. NC_007766.1.

3D structure databases

ProteinModelPortalQ2JZ21.
SMRQ2JZ21. Positions 121-731.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING347834.RHE_PF00275.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC94165; ABC94165; RHE_PF00275.
GeneID3895849.
KEGGret:RHE_PF00275.
PATRIC23092805. VBIRhiEtl108884_6031.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAHERCPHA.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycRETL347834:GJJ0-4419-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB2_RHIEC
AccessionPrimary (citable) accession number: Q2JZ21
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: March 7, 2006
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries