Reviewed, UniProtKB/Swiss-Prot Q2JZ21 (GLGB2_RHIEC)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme 2
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme 2
      Short name=BE 2
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2

Gene names

Name:	glgB2
Ordered Locus Names:	RHE_PF00275

Encoded on

Plasmid p42f

Organism

Rhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]

Taxonomic identifier

347834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	1,4-alpha-glucan-branching enzyme 2 HAMAP MF_00685		PRO_0000260683
Sites
	Active site	308	1	By similarity
	Active site	343	1	By similarity
	Active site	348	1	By similarity
	Active site	411	1	By similarity
	Active site	413	1	By similarity
	Active site	466	1	By similarity
	Active site	533	1	By similarity
	Active site	534	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2JZ21-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 23B9D25D7E41BA0E	FASTA	732	81,931
	10 20 30 40 50 60 MNVERSEFLA GVGHDALWAL IEGRHGDPFS ILGPHESGGM TIVRVYLPGA EGVDLIEAAS 70 80 90 100 110 120 GRVVTPFSIA HPSGLFAAAM GSRMHYRLRI TWPDGEQITE DPYSFGLLLG ELDLHLISEG 130 140 150 160 170 180 THYSLSRTLG AVEMAIDDVA GVRFAVWAPN ARRVSVVGDF NAWDGRRNPM RLRQSAGVWE 190 200 210 220 230 240 LFMPRLAPGE RYKFEIIDPH GNCLPQKADP VARASEAAPS TASIVASSTR FRWTDDNWMK 250 260 270 280 290 300 GQSRQQRLEG PISVYEVHAG SWLRENGGRS LDWVELSQRL VPYVREMGFT HIELLPIMEH 310 320 330 340 350 360 PFGGSWGYQP LGLFAPTGRY GTPEDLAYFI DRCHGAGIGV ILDWVPAHFP TDVWGLARFD 370 380 390 400 410 420 GTALYEHEDP REGFHRDWNT LIYNLGRNEV KGFLIASALE WLERYHIDGL RVDAVASMLY 430 440 450 460 470 480 RDYSRNEGEW IPNRYGGREN LEAVEFFKHL NSIVHERCPH AMMIAEESTA WPGVTKPPEE 490 500 510 520 530 540 GGLGFDMKWN MGWMHDSLSY IEKDPVYRSY HHGTMTFGMI YAYSERFILP ISHDEVVYGK 550 560 570 580 590 600 GSLLGKMPGD EWQKFANLRS YLAFMWGHPG KKLIFMGGEI AQPSEWNHDA SIAWDVLDQP 610 620 630 640 650 660 AHAGLQRLVK DLNGFYKDEA ALQFGDFHSE GFDWAAADDA VNSVLGMLRY APDRSSSVLV 670 680 690 700 710 720 VSNFTPVPRY GYRIGVPQDG VWIEKVTTDA REYGGSGLVN GAVSSESVPA HGRPHSLWLT 730 LPPLATVLLK SP

References

[1]

"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons." Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G. Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed: 16505379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000138 Genomic DNA. Translation: ABC94165.1.
RefSeq	YP_472892.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2JZ21.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	3895849.
GenomeReviews	Gene locus RHE_PF00275 in contig CP000138_GR.
KEGG	ret:RHE_PF00275.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2JZ21.
OMA	HERCPHA.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB2_RHIEC

Accession

Primary (citable) accession number: Q2JZ21

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)