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Protein

1,4-alpha-glucan branching enzyme GlgB 2

Gene

glgB2

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei413 – 4131NucleophileUniRule annotation
Active sitei466 – 4661Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-4419-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2UniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2UniRule annotation
Alpha-(1->4)-glucan branching enzyme 2UniRule annotation
Glycogen branching enzyme 2UniRule annotation
Short name:
BE 2UniRule annotation
Gene namesi
Name:glgB2UniRule annotation
Ordered Locus Names:RHE_PF00275
Encoded oniPlasmid p42f0 Publication
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000001936: Plasmid p42f

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7327321,4-alpha-glucan branching enzyme GlgB 2PRO_0000260683Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi347834.RHE_PF00275.

Structurei

3D structure databases

ProteinModelPortaliQ2JZ21.
SMRiQ2JZ21. Positions 121-731.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiQWIDAND.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2JZ21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVERSEFLA GVGHDALWAL IEGRHGDPFS ILGPHESGGM TIVRVYLPGA
60 70 80 90 100
EGVDLIEAAS GRVVTPFSIA HPSGLFAAAM GSRMHYRLRI TWPDGEQITE
110 120 130 140 150
DPYSFGLLLG ELDLHLISEG THYSLSRTLG AVEMAIDDVA GVRFAVWAPN
160 170 180 190 200
ARRVSVVGDF NAWDGRRNPM RLRQSAGVWE LFMPRLAPGE RYKFEIIDPH
210 220 230 240 250
GNCLPQKADP VARASEAAPS TASIVASSTR FRWTDDNWMK GQSRQQRLEG
260 270 280 290 300
PISVYEVHAG SWLRENGGRS LDWVELSQRL VPYVREMGFT HIELLPIMEH
310 320 330 340 350
PFGGSWGYQP LGLFAPTGRY GTPEDLAYFI DRCHGAGIGV ILDWVPAHFP
360 370 380 390 400
TDVWGLARFD GTALYEHEDP REGFHRDWNT LIYNLGRNEV KGFLIASALE
410 420 430 440 450
WLERYHIDGL RVDAVASMLY RDYSRNEGEW IPNRYGGREN LEAVEFFKHL
460 470 480 490 500
NSIVHERCPH AMMIAEESTA WPGVTKPPEE GGLGFDMKWN MGWMHDSLSY
510 520 530 540 550
IEKDPVYRSY HHGTMTFGMI YAYSERFILP ISHDEVVYGK GSLLGKMPGD
560 570 580 590 600
EWQKFANLRS YLAFMWGHPG KKLIFMGGEI AQPSEWNHDA SIAWDVLDQP
610 620 630 640 650
AHAGLQRLVK DLNGFYKDEA ALQFGDFHSE GFDWAAADDA VNSVLGMLRY
660 670 680 690 700
APDRSSSVLV VSNFTPVPRY GYRIGVPQDG VWIEKVTTDA REYGGSGLVN
710 720 730
GAVSSESVPA HGRPHSLWLT LPPLATVLLK SP
Length:732
Mass (Da):81,931
Last modified:March 7, 2006 - v1
Checksum:i23B9D25D7E41BA0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000138 Genomic DNA. Translation: ABC94165.1.
RefSeqiWP_011428582.1. NC_007766.1.
YP_472892.1. NC_007766.1.

Genome annotation databases

EnsemblBacteriaiABC94165; ABC94165; RHE_PF00275.
GeneIDi3895849.
KEGGiret:RHE_PF00275.
PATRICi23092805. VBIRhiEtl108884_6031.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000138 Genomic DNA. Translation: ABC94165.1.
RefSeqiWP_011428582.1. NC_007766.1.
YP_472892.1. NC_007766.1.

3D structure databases

ProteinModelPortaliQ2JZ21.
SMRiQ2JZ21. Positions 121-731.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi347834.RHE_PF00275.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC94165; ABC94165; RHE_PF00275.
GeneIDi3895849.
KEGGiret:RHE_PF00275.
PATRICi23092805. VBIRhiEtl108884_6031.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiQWIDAND.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciRETL347834:GJJ0-4419-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFN 42 / ATCC 51251.

Entry informationi

Entry nameiGLGB2_RHIEC
AccessioniPrimary (citable) accession number: Q2JZ21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: March 7, 2006
Last modified: March 4, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.