Q2JVC3 (GLO2_SYNJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	CYA_1132

Organism

Synechococcus sp. (strain JA-3-3Ab) (Cyanobacteria bacterium Yellowstone A-Prime) [Complete proteome] [HAMAP]

Taxonomic identifier

321327 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000068225
Sites
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	113	1	Zinc 1 By similarity
	Metal binding	132	1	Zinc 1 By similarity
	Metal binding	132	1	Zinc 2 By similarity
	Metal binding	170	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2JVC3 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: FA3D4D54015DE8A5	FASTA	252	27,825
	10 20 30 40 50 60 MRILQLPALR DNYIYLLHDP ETATAAVVDP TVAEPVLDKL AELGAELVAI FNTHHHHDHV 70 80 90 100 110 120 GGNLQLLARY PRAVVYGSQA DRGRIPGQTV ELQAGETVAF AGRQAKVLFV PGHTRGHVAY 130 140 150 160 170 180 YFPESGDLFC GDTLFAGGCG RLFEGTPEQM LGSLDQLRQL PEATRVWCAH EYTLNNLKFA 190 200 210 220 230 240 LTVDGDNPDL QARYQQVVAL RQAGSPTVPS TIGEERRTNP FLRWDQPALR AATGAQDPVR 250 VFARLRGMKD QF

References

[1]

"Population level functional diversity in a microbial community revealed by comparative genomic and metagenomic analyses." Bhaya D., Grossman A.R., Steunou A.-S., Khuri N., Cohan F.M., Hamamura N., Melendrez M.C., Bateson M.M., Ward D.M., Heidelberg J.F. ISME J. 1:703-713(2007) [PubMed: 18059494] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JA-3-3Ab.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000239 Genomic DNA. Translation: ABC99320.1.
RefSeq	YP_474583.1. NC_007775.1.
3D structure databases
HSSP	HSSP built from PDB template 2GCU based on UniProtKB Q9C8L4.
ProteinModelPortal	Q2JVC3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2JVC3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3898821.
GenomeReviews	Gene locus CYA_1132 in contig CP000239_GR.
KEGG	cya:CYA_1132.
PATRIC	23810535. VBISynSp90045_1122.
TIGR	CYA_1132.
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	IGCGRVF.
PhylomeDB	Q2JVC3.
ProtClustDB	CLSK750337.
Enzyme and pathway databases
BioCyc	SSP321327:CYA_1132-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_SYNJA

Accession

Primary (citable) accession number: Q2JVC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program