Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q2JV13 (SYL_SYNJA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CYA_1256
OrganismSynechococcus sp. (strain JA-3-3Ab) (Cyanobacteria bacterium Yellowstone A-Prime) [Complete proteome] [HAMAP]
Taxonomic identifier321327 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length873 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 873873Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009455

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif624 – 6285"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6271ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2JV13 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 14D89A03BC66321F

FASTA87398,433
        10         20         30         40         50         60 
MDARYNPPAI ESKWQAHWRE LGLDRTPELT AESRKFYALS MFPYPSGSLH MGHVRNYTIT 

        70         80         90        100        110        120 
DVIARHKRMQ GYAVLHPMGW DAFGLPAENA AIDRGIPPAK WTYQNIAQMR DQLQRLGLSY 

       130        140        150        160        170        180 
DWEREITTCA PDYYKWTQWL FLQFFKAGLA YQKEAPVNWD PVDQTVLANE QVDAEGRSWR 

       190        200        210        220        230        240 
SGALVEKRLL KQWFLKITAY ADQLLADLEK LSGWPERVLT MQENWIGQSV GARVVFKTET 

       250        260        270        280        290        300 
GEELPVFTTR PDTLWGATFM VLAPEHPLVE KLTTPEQEVA VKAYRAEAAA RSEIERSAED 

       310        320        330        340        350        360 
REKTGVWTGS YAINPVNQER IPIWIADYVL MGYGTGAIMA VPAHDQRDFE FARQFGLPIK 

       370        380        390        400        410        420 
LVVQPPQGGV TSAEDLQAAW TGEGVLINSG PLNGIPVGKG PGQSVERAIA WLEEQGLGER 

       430        440        450        460        470        480 
QVNYRLRDWL ISRQRYWGCP IPIIHCPHCG IVPVPEEDLP VLLPEDVELT GRGGSPLAQL 

       490        500        510        520        530        540 
EDWVKVKCPA CGADARRETD TMDTFICSSW YFLRFSDPRN DREIFRKDLV NAWLPVDQYV 

       550        560        570        580        590        600 
GGIEHAILHL LYSRFFTKVL RDLGLLNFDE PFSRLLTQGM VQARTYYNPN KSGKDRWIPT 

       610        620        630        640        650        660 
ALVKDPNDPR DPETGEPLQV IYATMSKSKG NGVDPEEVLA NYGADTARMF ILFKAPPEKD 

       670        680        690        700        710        720 
LEWDDADVEG QFRFLNRVWR QVYEFVVRGS GTESLQGKAA ELLQAKVEAG SLTKAERDLR 

       730        740        750        760        770        780 
RAVHTAIKEV SEDLEEYQFN TAIAGLMKLS NALAEAEIPD SPVYAEGIRT LVLLLAPFAP 

       790        800        810        820        830        840 
HMAEELWQAL GGTDSVHRQA WPTYDPAALV ADTVTIVVQV NGKLRGSFEA PADVTPQEQE 

       850        860        870 
RLALQSEAAQ RYLQGMTPKR VIVVPKKLVN LVV 

« Hide

References

[1]"Population level functional diversity in a microbial community revealed by comparative genomic and metagenomic analyses."
Bhaya D., Grossman A.R., Steunou A.-S., Khuri N., Cohan F.M., Hamamura N., Melendrez M.C., Bateson M.M., Ward D.M., Heidelberg J.F.
ISME J. 1:703-713(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JA-3-3Ab.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000239 Genomic DNA. Translation: ABC99439.1.
RefSeqYP_474702.1. NC_007775.1.

3D structure databases

ProteinModelPortalQ2JV13.
SMRQ2JV13. Positions 4-809.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING321327.CYA_1256.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC99439; ABC99439; CYA_1256.
GeneID3899407.
KEGGcya:CYA_1256.
PATRIC23810792. VBISynSp90045_1247.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSSP321327:GHFX-1250-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SYNJA
AccessionPrimary (citable) accession number: Q2JV13
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries