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Q2JR27 (Q2JR27_SYNJA) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168 RuleBase RU003777
EC=2.4.2.29 HAMAP-Rule MF_00168 RuleBase RU003777
Alternative name(s):
Guanine insertion enzyme HAMAP-Rule MF_00168
tRNA-guanine transglycosylase HAMAP-Rule MF_00168
Gene names
Name:tgt HAMAP-Rule MF_00168
Ordered Locus Names:CYA_2841
OrganismSynechococcus sp. (strain JA-3-3Ab) (Cyanobacteria bacterium Yellowstone A-Prime) [Complete proteome] [HAMAP] EMBL ABD00942.1
Taxonomic identifier321327 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168 SAAS SAAS004803 RuleBase RU003777

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. RuleBase RU003777

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00168 SAAS SAAS004803 RuleBase RU003777

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168 SAAS SAAS004803

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. HAMAP-Rule MF_00168 RuleBase RU003777

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site941Nucleophile By similarity HAMAP-Rule MF_00168
Metal binding3051Zinc By similarity HAMAP-Rule MF_00168
Metal binding3071Zinc By similarity HAMAP-Rule MF_00168
Metal binding3101Zinc By similarity HAMAP-Rule MF_00168
Metal binding3361Zinc By similarity HAMAP-Rule MF_00168
Binding site951Substrate By similarity HAMAP-Rule MF_00168

Sequences

Sequence LengthMass (Da)Tools
Q2JR27 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 673CCF4A5F6B8051

FASTA36740,806
        10         20         30         40         50         60 
MAAFTFVCEG TCPNTQARAG YFITPHGEIP TPCFMPVGTL ANVKTLTPAQ LKETGAPILL 

        70         80         90        100        110        120 
CNTYHLHLQP GESIIQEAGG LHHFMGWPGP ILTDSGGFQV FSLSAIRKID EEGVTFRSPY 

       130        140        150        160        170        180 
DGRLVRFTPE LAIQIQNALG ADIIMAFDEC PPYPASPEAV AAAVRRTNQW LKRCVEAHRR 

       190        200        210        220        230        240 
SDQALFGIVQ GGVYPELRHQ AVEEVCRLDL PGYAIGGVSV GEPVELMHAV VRQTAPLLPA 

       250        260        270        280        290        300 
DRPRYLMGVG TYAEMRAAVA AGVDLFDCVI PTRYARHGTV ISRGKRWNIK NAEHRRSLDP 

       310        320        330        340        350        360 
IDPDCPCYTC RHFSRAYLCH LIRAGELLGY TLLSIHNITE LVRYMQKMRQ AILEGKFHEN 


WDPWREG

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References

[1]"Population level functional diversity in a microbial community revealed by comparative genomic and metagenomic analyses."
Bhaya D., Grossman A.R., Steunou A.-S., Khuri N., Cohan F.M., Hamamura N., Melendrez M.C., Bateson M.M., Ward D.M., Heidelberg J.F.
ISME J. 1:703-713(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JA-3-3Ab.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000239 Genomic DNA. Translation: ABD00942.1.
RefSeqYP_476205.1. NC_007775.1.

3D structure databases

ProteinModelPortalQ2JR27.
ModBaseSearch...

Protein-protein interaction databases

STRING321327.CYA_2841.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD00942; ABD00942; CYA_2841.
GeneID3898936.
KEGGcya:CYA_2841.
PATRIC23813928. VBISynSp90045_2786.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMAECIPYPC.
ProtClustDBCLSK750790.

Enzyme and pathway databases

UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ2JR27_SYNJA
AccessionPrimary (citable) accession number: Q2JR27
Entry history
Integrated into UniProtKB/TrEMBL: March 7, 2006
Last sequence update: March 7, 2006
Last modified: May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info