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Protein

Nitrogenase iron protein

Gene

nifH

Organism
Synechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.UniRule annotation

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi96Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi130Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron proteinUniRule annotation (EC:1.18.6.1UniRule annotation)
Alternative name(s):
Nitrogenase Fe proteinUniRule annotation
Nitrogenase component IIUniRule annotation
Nitrogenase reductaseUniRule annotation
Gene namesi
Name:nifHUniRule annotation
Ordered Locus Names:CYB_0421
OrganismiSynechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime)
Taxonomic identifieri321332 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000001938 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002118911 – 292Nitrogenase iron proteinAdd BLAST292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei99ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseUniRule annotation1

Post-translational modificationi

The reversible ADP-ribosylation of Arg-99 inactivates the nitrogenase reductase and regulates nitrogenase activity.UniRule annotation

Keywords - PTMi

ADP-ribosylation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi321332.CYB_0421.

Structurei

3D structure databases

ProteinModelPortaliQ2JP78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228826.
KOiK02588.
OMAiNKMFVIP.
OrthoDBiPOG091H0230.

Family and domain databases

CDDicd02040. NifH. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2JP78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQIAFYGKG GIGKSTTCQN TVAGMAELGQ RIMIVGCDPK ADSTRLMLHC
60 70 80 90 100
KAQTTVLHLA AERGSVEDVE LEEVVLTGYR GVRCVESGGP EPGVGCAGRG
110 120 130 140 150
IITAINFLEE NGAYEDLDFV CYDVLGDVVC GGFAMPIREG KAQEIYIVCS
160 170 180 190 200
GEMMAMYAAN NIARGVLKYA YSGGVRLGGL ICNSRKVDRE IELIEALAEK
210 220 230 240 250
LNTKMLHFIP RDNVVQHAEL RRMTVIEYSP DCNQADEYRA LAKKIINNTD
260 270 280 290
LRIPTPISMD ELEQLLIEFG VLDDDQKIAH LIGKTEKELA PV
Length:292
Mass (Da):31,978
Last modified:March 7, 2006 - v1
Checksum:i71FE840015DB6FDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000240 Genomic DNA. Translation: ABD01417.1.
RefSeqiWP_011430651.1. NC_007776.1.

Genome annotation databases

EnsemblBacteriaiABD01417; ABD01417; CYB_0421.
KEGGicyb:CYB_0421.
PATRICi23803130. VBISynSp29577_0431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000240 Genomic DNA. Translation: ABD01417.1.
RefSeqiWP_011430651.1. NC_007776.1.

3D structure databases

ProteinModelPortaliQ2JP78.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi321332.CYB_0421.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD01417; ABD01417; CYB_0421.
KEGGicyb:CYB_0421.
PATRICi23803130. VBISynSp29577_0431.

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228826.
KOiK02588.
OMAiNKMFVIP.
OrthoDBiPOG091H0230.

Family and domain databases

CDDicd02040. NifH. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIFH_SYNJB
AccessioniPrimary (citable) accession number: Q2JP78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 7, 2006
Last modified: November 2, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.