Q2JLY8 (LPXD_SYNJB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	CYB_1289

Organism

Synechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime) [Complete proteome] [HAMAP]

Taxonomic identifier

321332 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000264445
Sites
	Active site	237	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2JLY8 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 1C7A5D2E2A94DBC3	FASTA	363	38,862
	10 20 30 40 50 60 MQLQEIAQKL GCAYEGDPTL EIHSVASLAE ARPGELSFLS EARYLPLLEQ TQASAVIVEE 70 80 90 100 110 120 GLALPCSIAC LRGRDPRLLF AQAIELFYQP YRLPVGIHPT AVIDPSVELG EGVAIGPHAV 130 140 150 160 170 180 VMEGVKIGDH TQIHPNVTIY PHVRIGSRCQ LFANCVIHER TEIGDDCLIH SGAVIGDDGF 190 200 210 220 230 240 GHIPLADGSW RRMLQAGRVV LEDNVEVGSN TTIDRAAVGE TRIGRGTKID NLVQIGHGVR 250 260 270 280 290 300 TGSHCLIVAQ VGIAGSTQLG HHVILAGQCG LAGHLHIGDG VRVAAQTGVT SDVPAGQTVA 310 320 330 340 350 360 GYPHQPIAEW RKSMAVQRHL PELQRTLRKL EARVAKLEQN STDRAPNAKM LEVGVDPETT CSS

References

[1]

"Population level functional diversity in a microbial community revealed by comparative genomic and metagenomic analyses." Bhaya D., Grossman A.R., Steunou A.-S., Khuri N., Cohan F.M., Hamamura N., Melendrez M.C., Bateson M.M., Ward D.M., Heidelberg J.F. ISME J. 1:703-713(2007) [PubMed: 18059494] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JA-2-3B'a(2-13).

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000240 Genomic DNA. Translation: ABD02263.1.
RefSeq	YP_477526.1. NC_007776.1.
3D structure databases
ProteinModelPortal	Q2JLY8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2JLY8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3901023.
GenomeReviews	Gene locus CYB_1289 in contig CP000240_GR.
KEGG	cyb:CYB_1289.
PATRIC	23804892. VBISynSp29577_1299.
TIGR	CYB_1289.
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	SYPAKIM.
PhylomeDB	Q2JLY8.
Enzyme and pathway databases
BioCyc	SSP321332:CYB_1289-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 6 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_SYNJB

Accession

Primary (citable) accession number: Q2JLY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program