Q2JKY3 (SYE_SYNJB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	CYB_1680

Organism

Synechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime) [Complete proteome] [HAMAP]

Taxonomic identifier

321332 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000237411
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	243 – 247	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	246	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2JKY3 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 6291942B0C9A6D7B	FASTA	479	53,767
	10 20 30 40 50 60 MSVCVRIAPS PTGNLHIGTA RTAVFNWLYA RRHGGRFILR IEDTDRDRSL PRYTRNILAG 70 80 90 100 110 120 LAWLGLDWDE GPVYQSKRIE RYQAVVQQLL DRGLAYRCYV SEAELEEMRA AQKAAGKAPR 130 140 150 160 170 180 YDNRHRFLTE AQRRAYEAEG RQPVIRFKIE EPLEVSWVDL IRGPITWNTQ DLGGDMVIAR 190 200 210 220 230 240 ADGCPLYNLA VVVDDIDMGI THVIRGEDHI GNTPKQILLY RALGHEPPQF AHSPLILNPE 250 260 270 280 290 300 GKKLSKRDGA TSVAEFQQLG FLPEALKNYL ALLSWSPPDG EELFSLEKAA ALFDFDRVNR 310 320 330 340 350 360 AAARFDWDKL NWINSQYIKR LSPPELVERL TPFWQAAGFD LSEVPDPTWL EDVARLIADG 370 380 390 400 410 420 IDRLAEAPPL SRFLFQEPLS YTLPALEQLR LPGVAEAMAA MATTLAAAEL PQVSADSLKP 430 440 450 460 470 LVDQVAKGQN MKKGLLMKSL RAALTGDLQG PDLLESFALL QRRGWALGRL EAVQKLVPC

References

[1]

"Population level functional diversity in a microbial community revealed by comparative genomic and metagenomic analyses." Bhaya D., Grossman A.R., Steunou A.-S., Khuri N., Cohan F.M., Hamamura N., Melendrez M.C., Bateson M.M., Ward D.M., Heidelberg J.F. ISME J. 1:703-713(2007) [PubMed: 18059494] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JA-2-3B'a(2-13).

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000240 Genomic DNA. Translation: ABD02639.1.
RefSeq	YP_477902.1. NC_007776.1.
3D structure databases
ProteinModelPortal	Q2JKY3.
SMR	Q2JKY3. Positions 2-474.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2JKY3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3901895.
GenomeReviews	Gene locus CYB_1680 in contig CP000240_GR.
KEGG	cyb:CYB_1680.
PATRIC	23805688. VBISynSp29577_1686.
TIGR	CYB_1680.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	ESIIQFV.
PhylomeDB	Q2JKY3.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	SSP321332:CYB_1680-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_SYNJB

Accession

Primary (citable) accession number: Q2JKY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program