Q2JKE8 (METE_SYNJB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	CYB_1886

Organism

Synechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime) [Complete proteome] [HAMAP]

Taxonomic identifier

321332 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	754 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 754	754	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017287
Sites
	Metal binding	631	1	Zinc By similarity
	Metal binding	633	1	Zinc By similarity
	Metal binding	716	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2JKE8 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: EAD695787C51316B	FASTA	754	84,676
	10 20 30 40 50 60 MTAQTMTLGY ARMGKRRELK KALEGFWSGA LGSEALLATF WDLETQAWQT QLQAGIDHIA 70 80 90 100 110 120 VGDQTLYDHV LDWATWLGLI PSRFRGLSGL DRYFAMARGR EGLPALEMTK WFDTNYHYLV 130 140 150 160 170 180 PEIEPEADPS PNFGDFLERV RRAQGILGER TSPVVLSPVT LLCLSQRSGD LRADLEKLLP 190 200 210 220 230 240 LYRDLLQELK QLGIPEVQIH DPILVTSQGS GLREAVEMSY RQLATAGIPV HLVTYFDDLG 250 260 270 280 290 300 ETYPWVVQLP VAGISLDFTR GHTLDLVRTY GFPADQILGA GVVDARNVWK VQPETVLASL 310 320 330 340 350 360 RELQGVAPNL RVQPSASLQF VPHDAALEAQ LPEPLRNVLS FAEQKLAEVA LLARALNGED 370 380 390 400 410 420 TAAQQAEIQQ QWQSFAQFSP PNPQVRQALA NLKLQDFERF LPYEQRLSRQ VQLPPLPTTT 430 440 450 460 470 480 IGSFPQTPEV RQWRAKYKRG EVSQAEYEAA IDAEIAKCIR IQEEIGLDVL VHGEFERTDM 490 500 510 520 530 540 VEYFAQKLEG FAFTEHGWVQ SYGSRCVRPP ILYGDVVRPQ PMTVREFQVA QSHTQKPVKG 550 560 570 580 590 600 MLTGPVTMLN WSFPRVDIPR REQALQIALA LRAEVADLEA AGAVMVQVDE PALREGLPLK 610 620 630 640 650 660 KERWPEYLSW AVDAFRLATG GAKPETQIHT HMCYSEFGDI IEHIERLDAD VLSIENSRSN 670 680 690 700 710 720 NKTLLQIAQA GYRHQVGNGV YDVHSPAVPS VEQILQQLRT GLAHLPVEQT WVNPDCGLKT 730 740 750 RRWEEVIPAL KNMVAAAHQL GEELLETQPE GLRE

References

[1]

"Population level functional diversity in a microbial community revealed by comparative genomic and metagenomic analyses." Bhaya D., Grossman A.R., Steunou A.-S., Khuri N., Cohan F.M., Hamamura N., Melendrez M.C., Bateson M.M., Ward D.M., Heidelberg J.F. ISME J. 1:703-713(2007) [PubMed: 18059494] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JA-2-3B'a(2-13).

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000240 Genomic DNA. Translation: ABD02841.1.
RefSeq	YP_478104.1. NC_007776.1.
3D structure databases
ProteinModelPortal	Q2JKE8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2JKE8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3900829.
GenomeReviews	Gene locus CYB_1886 in contig CP000240_GR.
KEGG	cyb:CYB_1886.
PATRIC	23806110. VBISynSp29577_1894.
TIGR	CYB_1886.
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	NIWANDF.
PhylomeDB	Q2JKE8.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	SSP321332:CYB_1886-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_SYNJB

Accession

Primary (citable) accession number: Q2JKE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program