Q2JK60 (ILVD_SYNJB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	CYB_1987

Organism

Synechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime) [Complete proteome] [HAMAP]

Taxonomic identifier

321332 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP-Rule MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP-Rule MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP-Rule MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Dihydroxy-acid dehydratase HAMAP-Rule MF_00012		PRO_0000321608
Sites
	Metal binding	126	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	198	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2JK60 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 3CB28147B1DD4C6C	FASTA	565	59,804
	10 20 30 40 50 60 MANSPFPLRS QVVTQGVQRS PNRAMLRAVG FRDEDFGKPI VGIANAHSTL TPCNMGIQTL 70 80 90 100 110 120 AERAEAALRT AGCMPQVFGT ITISDGISMG TEGMKYSLVS REVIADSIET VVNGQSMDGL 130 140 150 160 170 180 LAIGGCDKNM PGAMIAMARL NVPSIFVYGG TIKPGHYNGR DLTIVSAFEA VGEFSAGRIS 190 200 210 220 230 240 EEELLAVERH ACPGAGSCGG MYTANTMSSA FEAMGMSLPY SSTMAAEDEE KAESAAQSAK 250 260 270 280 290 300 VLAEAIKANI RPRDIITRKS IENAISVIMA VGGSTNAVLH FLAIAHAAEV PLTLDDFETI 310 320 330 340 350 360 RARVPVLCDL KPSGRFVATD LHRAGGIPQV MKMLLNHGLL HGDCLTISGQ TIVEVLRDVP 370 380 390 400 410 420 DEPSPDQEVI RPWHSPLYPQ GHLAILKGNL APEGAVAKIT GVKNPQITGP ARVFDSEESC 430 440 450 460 470 480 LQAILSGQIR AGDVVVIRYE GPKGGPGMRE MLSPTSAIIG AGLGDSVGLI TDGRFSGGTY 490 500 510 520 530 540 GMVVGHVAPE AYVGGTIALV EEGDLITIDA PARQLHLHVS EEELARRRAR WSPPEPRYKR 550 560 GTLAKYAKLV SSSSLGAVTD LNLWD

References

[1]

"Population level functional diversity in a microbial community revealed by comparative genomic and metagenomic analyses." Bhaya D., Grossman A.R., Steunou A.-S., Khuri N., Cohan F.M., Hamamura N., Melendrez M.C., Bateson M.M., Ward D.M., Heidelberg J.F. ISME J. 1:703-713(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JA-2-3B'a(2-13).

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000240 Genomic DNA. Translation: ABD02935.1.
RefSeq	YP_478198.1. NC_007776.1.
3D structure databases
ProteinModelPortal	Q2JK60.
ModBase	Search...
Protein-protein interaction databases
STRING	321332.CYB_1987.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD02935; ABD02935; CYB_1987.
GeneID	3900000.
KEGG	cyb:CYB_1987.
PATRIC	23806322. VBISynSp29577_1995.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HOG000173155.
KO	K01687.
OMA	NMPGAMI.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	SSP321332:GH1B-1987-MONOMER.
UniPathway	UPA00047; UER00057. UPA00049; UER00061.
Family and domain databases
HAMAP	MF_00012. IlvD.
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. PTHR21000. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_SYNJB

Accession

Primary (citable) accession number: Q2JK60

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 7, 2006
Last modified:	May 29, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program