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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Synechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72S-adenosyl-L-methionineUniRule annotation1
Binding sitei97S-adenosyl-L-methionineUniRule annotation1
Binding sitei124S-adenosyl-L-methionineUniRule annotation1
Active sitei147By similarity1
Binding sitei147S-adenosyl-L-methionineUniRule annotation1
Binding sitei151SubstrateUniRule annotation1
Binding sitei183SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:CYB_2166
OrganismiSynechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime)
Taxonomic identifieri321332 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000001938 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002882451 – 239tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST239

Interactioni

Protein-protein interaction databases

STRINGi321332.CYB_2166.

Structurei

3D structure databases

ProteinModelPortaliQ2JJQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107YD0. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000237936.
KOiK03439.
OMAiFERIYIK.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2JJQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAEVQQGQQ SPEGQLEKRP PSPPWAGIPL QRGLGHRVRQ HVNPLQAQYQ
60 70 80 90 100
QPAPPPHWER VYRRLGQPFH LDIGTGSGRF LLRIAQEQPD WNFLGVEIRQ
110 120 130 140 150
ALVERANAWR DELGLDNVHF LFANINVSLR HLFAPGDLSR VTILFPDPWF
160 170 180 190 200
KKRHHKRRIV QPQLVADLAL LLRPGSPVFL QSDIQEVAEE MAARFLEHPQ
210 220 230
FWDPHQGPLD SNPLGIPTER EWQCLQLDLP IYRYWLERR
Length:239
Mass (Da):27,864
Last modified:March 7, 2006 - v1
Checksum:iD38937C1837D1DC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000240 Genomic DNA. Translation: ABD03112.1.
RefSeqiWP_011433747.1. NC_007776.1.

Genome annotation databases

EnsemblBacteriaiABD03112; ABD03112; CYB_2166.
KEGGicyb:CYB_2166.
PATRICi23806688. VBISynSp29577_2177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000240 Genomic DNA. Translation: ABD03112.1.
RefSeqiWP_011433747.1. NC_007776.1.

3D structure databases

ProteinModelPortaliQ2JJQ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi321332.CYB_2166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD03112; ABD03112; CYB_2166.
KEGGicyb:CYB_2166.
PATRICi23806688. VBISynSp29577_2177.

Phylogenomic databases

eggNOGiENOG4107YD0. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000237936.
KOiK03439.
OMAiFERIYIK.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_SYNJB
AccessioniPrimary (citable) accession number: Q2JJQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 7, 2006
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.