Q2JJN4 (PIMT_SYNJB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-L-isoaspartate O-methyltransferase
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Short name=PIMT

Gene names

Name:	pcm
Ordered Locus Names:	CYB_2184

Organism

Synechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime) [Complete proteome] [HAMAP]

Taxonomic identifier

321332 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity. HAMAP-Rule MF_00090
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP-Rule MF_00090
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Protein-L-isoaspartate O-methyltransferase HAMAP-Rule MF_00090		PRO_0000351943
Sites
	Active site	92	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2JJN4 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: C3BD423D67824840	FASTA	244	26,518
	10 20 30 40 50 60 MINPFSSFRW RHSSRSPAGI PEVEPQPPDA SDPFASQREQ MVKKQLIKRG IRDPRVLEAM 70 80 90 100 110 120 GKVPRHLFVP SDLRDRAYRD CPLPIGYGQT ISQPYIVAFM TEAARLTPES VVLEIGTGSG 130 140 150 160 170 180 YQAAVLAELA RQVYSLERLS PLAAQAQQTL AALGYRNVEV RQGDGYQGWP EHAPYDAILV 190 200 210 220 230 240 TAAPPTVPMV LLDQLAVGGT LVVPVGESPS DSGAQGSQSL LILCKTAQGW VQKGAFPVQF VPMV

References

[1]

"Population level functional diversity in a microbial community revealed by comparative genomic and metagenomic analyses." Bhaya D., Grossman A.R., Steunou A.-S., Khuri N., Cohan F.M., Hamamura N., Melendrez M.C., Bateson M.M., Ward D.M., Heidelberg J.F. ISME J. 1:703-713(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JA-2-3B'a(2-13).

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000240 Genomic DNA. Translation: ABD03128.1.
RefSeq	YP_478391.1. NC_007776.1.
3D structure databases
ProteinModelPortal	Q2JJN4.
ModBase	Search...
Protein-protein interaction databases
STRING	321332.CYB_2184.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD03128; ABD03128; CYB_2184.
GeneID	3902885.
KEGG	cyb:CYB_2184.
PATRIC	23806720. VBISynSp29577_2192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2518.
HOGENOM	HOG000257189.
KO	K00573.
OMA	DGYQGWP.
ProtClustDB	CLSK844542.
Family and domain databases
HAMAP	MF_00090. PIMT.
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PTHR11579. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_SYNJB

Accession

Primary (citable) accession number: Q2JJN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	March 7, 2006
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program