Q2JI26 (MNMG_SYNJB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	CYB_2823

Organism

Synechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime) [Complete proteome] [HAMAP]

Taxonomic identifier

321332 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00129
Cofactor	FAD By similarity. HAMAP-Rule MF_00129
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP-Rule MF_00129
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00129.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129		PRO_0000345345
Regions
	Nucleotide binding	21 – 26	6	FAD By similarity
	Nucleotide binding	282 – 296	15	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2JI26 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 4D3C7BF39A585F2A	FASTA	643	71,885
	10 20 30 40 50 60 MLRVGRDSID FLDHYDVVVV GGGHSGCEAA LAAARLGCNT LMLTLNLDKI AWQPCNPAVG 70 80 90 100 110 120 GPAKSQLVHE IDALGGEMGK VTDRTYLQKR VLNRSRGPAV WALRAQTDKR EYARVMRSVV 130 140 150 160 170 180 ENQPNLTIRE GTVTDLVLGR NDEVVGVVTH FGTIFGCRAV ILTTGTFLGG RIWIGRHWQA 190 200 210 220 230 240 AGRAGEFAVE GLTDTLRQLG FETGRLKTGT PARVDRRSVD FSVMERQPGD PDVRWFSFDP 250 260 270 280 290 300 EVWVPREQMD CYLTRTTPET HRIIRENLHE TPVYGGWVEA KGPRYCPSIE DKIVRFADKE 310 320 330 340 350 360 SHQIFIEPEG RDLPELYIQG FSTGMPEKIQ IQMLRSLPGL ERCVMLRPAY AVEYDYLPAT 370 380 390 400 410 420 QLYPTLMTKK VQGLFCAGQI NGTTGYEEAA AQGLIAGINA ARLVQGKPLV TLPRESSYIG 430 440 450 460 470 480 TLIDDLCTKE LREPYRMLTS RSEYRLILRS DNADQRLTPL GREWGLIDDR RWALFQAKQA 490 500 510 520 530 540 RIAAEIERLE TQRVKAHDLA GIQLSQLTGQ GIKGSATLAE ILRRNQIHYS DLLELGLGDA 550 560 570 580 590 600 ELDPFEQEAA EIAVKYSGYI QRQQSQIEQV SKQYHRPLPP DLDYHSIPTL SKESRDKLSA 610 620 630 640 VRPLTVGQAA RIGGVNPADI NALLIYLEVR QRQRAADPVC SSL

References

[1]

"Population level functional diversity in a microbial community revealed by comparative genomic and metagenomic analyses." Bhaya D., Grossman A.R., Steunou A.-S., Khuri N., Cohan F.M., Hamamura N., Melendrez M.C., Bateson M.M., Ward D.M., Heidelberg J.F. ISME J. 1:703-713(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JA-2-3B'a(2-13).

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000240 Genomic DNA. Translation: ABD03745.1.
RefSeq	YP_479008.1. NC_007776.1.
3D structure databases
ProteinModelPortal	Q2JI26.
ModBase	Search...
Protein-protein interaction databases
STRING	321332.CYB_2823.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD03745; ABD03745; CYB_2823.
GeneID	3900768.
KEGG	cyb:CYB_2823.
PATRIC	23808018. VBISynSp29577_2830.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0445.
HOGENOM	HOG000201060.
KO	K03495.
OMA	AQMSCNP.
ProtClustDB	PRK05192.
Enzyme and pathway databases
BioCyc	SSP321332:GH1B-2822-MONOMER.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA.
InterPro	IPR004416. GidA. IPR026904. GidA-assoc_3. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. PF13932. GIDA_assoc_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. gidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_SYNJB

Accession

Primary (citable) accession number: Q2JI26

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	March 7, 2006
Last modified:	May 29, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program