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Q2JHX0 (SYL_SYNJB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CYB_2881
OrganismSynechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime) [Complete proteome] [HAMAP]
Taxonomic identifier321332 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length882 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 882882Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009456

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif632 – 6365"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6351ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2JHX0 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 6ED220A8B591A861

FASTA88299,521
        10         20         30         40         50         60 
MDARYNPQAI ESKWQAHWRE IGLDRTPELK DGSRKFYALS MFPYPSGNLH MGHVRNYTIT 

        70         80         90        100        110        120 
DVIARHKRMQ GYAVLHPMGW DAFGLPAENA AIDRGIPPAK WTYQNIAQMR EQLQRLGLSY 

       130        140        150        160        170        180 
DWEREITTCA PDYYKWTQWL FLQFFKAGLA YQKEAPVNWD PVDQTVLANE QVDAEGRSWR 

       190        200        210        220        230        240 
SGALVEKRML KQWFFKITAY ADQLLADLEK LSGWPERVRT MQENWIGQSV GAKVIFKTEA 

       250        260        270        280        290        300 
GDELAVFTTR PDTLWGATFM VMSPEHPLVD KLTTAEQLQA VRAYQAQAAA RSEIERSAED 

       310        320        330        340        350        360 
REKTGVWTGS YAINPVNQER IPIWIADYVL MGYGTGAIMA VPAHDQRDFE FARKFGLPIK 

       370        380        390        400        410        420 
RVVQPPEGSL SSTDRASGTE SSELQAAWTG EGVMINSGPL DGIPVGKGPG QSVERAIAWL 

       430        440        450        460        470        480 
EAQGLGEKQI NYRLRDWLIS RQRYWGCPIP VIHCPHCGIV PVPEKDLPVL LPEDVELTGR 

       490        500        510        520        530        540 
GGSPLAQLED WVKVKCPTCG AEARRETDTM DTFICSSWYF LRFSDARNDR EIFRKDRVNA 

       550        560        570        580        590        600 
WLPVDQYVGG IEHAILHLLY SRFFTKVLRD RGLLDFDEPF LRLLTQGMVQ GRTYYNPNKS 

       610        620        630        640        650        660 
GKDRWIPAAL VKDPDNPTDP ETGEPLEVIY ATMSKSKGNG VDPEEVLAHY GADTARMFIL 

       670        680        690        700        710        720 
FKAPPEKDLE WDDADVEGQF RFLNRVWRQV YEFVVRGGGT ESWRGRVSEL LPAKVEVGSL 

       730        740        750        760        770        780 
TKAEKDLRRA IHTAIKEVSE DLENDYQFNT AIAELMKLSN ALGEAGIPDS PVYAEGIRTL 

       790        800        810        820        830        840 
VLLMAPFAPH IAEELWQALG GADSVHRQSW PSYDPAALVA DTVTIVIQVN GKLRGSFEAP 

       850        860        870        880 
AEVTPEEQEQ LALRSEAAQK YLEGATPKKV VVVPKKLVNF VL 

« Hide

References

[1]"Population level functional diversity in a microbial community revealed by comparative genomic and metagenomic analyses."
Bhaya D., Grossman A.R., Steunou A.-S., Khuri N., Cohan F.M., Hamamura N., Melendrez M.C., Bateson M.M., Ward D.M., Heidelberg J.F.
ISME J. 1:703-713(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JA-2-3B'a(2-13).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000240 Genomic DNA. Translation: ABD03801.1.
RefSeqYP_479064.1. NC_007776.1.

3D structure databases

ProteinModelPortalQ2JHX0.
SMRQ2JHX0. Positions 4-818.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING321332.CYB_2881.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD03801; ABD03801; CYB_2881.
GeneID3900583.
KEGGcyb:CYB_2881.
PATRIC23808132. VBISynSp29577_2887.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAVHRQSWP.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSSP321332:GH1B-2881-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SYNJB
AccessionPrimary (citable) accession number: Q2JHX0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries