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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Frankia sp. (strain CcI3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 3011D-inositol 3-phosphateUniRule annotation
Binding sitei44 – 441UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei99 – 9911D-inositol 3-phosphateUniRule annotation
Binding sitei132 – 13211D-inositol 3-phosphateUniRule annotation
Binding sitei156 – 15611D-inositol 3-phosphateUniRule annotation
Binding sitei176 – 17611D-inositol 3-phosphateUniRule annotation
Binding sitei250 – 2501UDP-GlcNAcUniRule annotation
Binding sitei255 – 2551UDP-GlcNAcUniRule annotation
Metal bindingi325 – 3251Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi326 – 3261Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi328 – 3281Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei338 – 3381UDP-GlcNAcUniRule annotation
Binding sitei346 – 3461UDP-GlcNAcUniRule annotation
Metal bindingi352 – 3521MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciFSP106370:GI1F-469-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:Francci3_0456
OrganismiFrankia sp. (strain CcI3)
Taxonomic identifieri106370 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000001937 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435D-inositol 3-phosphate glycosyltransferasePRO_0000400123Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi106370.Francci3_0456.

Structurei

3D structure databases

ProteinModelPortaliQ2JFV0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 372UDP-GlcNAc bindingUniRule annotation
Regioni41 – 4661D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2JFV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLIRSGAAQ VRGEAERRGR PSRVAMLSMH TSPMEQPGTG DAGGLNVYVV
60 70 80 90 100
ELSRQLAALG VEVEVFTRAV SSKLPTSAEL LPGVTVRHVD AGPFEEIHRE
110 120 130 140 150
DLPAWLCAFT AALLRAEAGH EPGWFDVIHS HYWLSGQVGL AVAQRWGIPL
160 170 180 190 200
VHTSHTLAKI KNGALAVGDR PEPPGRLLGE QEVIGGATRL LASTPDEYRH
210 220 230 240 250
LIDLYDAASD RVDVVAPGVD LEVFRPGDMA QSRARVGVDP ADDLLLFVGR
260 270 280 290 300
IQPLKAPDLL LRAAAELLRR DPARRSRLTV AVVGGPSGSG LEQPDALVKL
310 320 330 340 350
AAYLGISDRV RFQPPAPQQE LVHWYRAATA VVVPSHSESF GLVALEAQAC
360 370 380 390 400
GTPVVAAAVG GLRTAVADGV SGLLVSGRTP AVYADALDRL LRQPRWRARL
410 420 430
SAGAVAWAGG FGWSATAHGV LRSYRHALSP TAVAV
Length:435
Mass (Da):46,275
Last modified:March 7, 2006 - v1
Checksum:i2D226ED3F72607A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD09842.1.
RefSeqiWP_011434919.1. NC_007777.1.

Genome annotation databases

EnsemblBacteriaiABD09842; ABD09842; Francci3_0456.
KEGGifra:Francci3_0456.
PATRICi21923756. VBIFraSp10456_0506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD09842.1.
RefSeqiWP_011434919.1. NC_007777.1.

3D structure databases

ProteinModelPortaliQ2JFV0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi106370.Francci3_0456.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD09842; ABD09842; Francci3_0456.
KEGGifra:Francci3_0456.
PATRICi21923756. VBIFraSp10456_0506.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Enzyme and pathway databases

BioCyciFSP106370:GI1F-469-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CcI3.

Entry informationi

Entry nameiMSHA_FRASC
AccessioniPrimary (citable) accession number: Q2JFV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 7, 2006
Last modified: May 11, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.