Q2JFB3 (Q2JFB3_FRASC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344
EC=6.3.5.2 HAMAP MF_00344

Alternative name(s):
GMP synthetase HAMAP MF_00344
Glutamine amidotransferase HAMAP MF_00344

Gene names

Name:	guaA HAMAP MF_00344
Ordered Locus Names:	Francci3_0645

Organism

Frankia sp. (strain CcI3) [Complete proteome] [HAMAP] EMBL ABD10029.1

Taxonomic identifier

106370 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 GMP-binding domain. HAMAP MF_00344 Contains 1 glutamine amidotransferase type-1 domain. HAMAP MF_00344

Ontologies

Keywords
Biological process	GMP biosynthesis HAMAP MF_00344 Purine biosynthesis
Domain	Glutamine amidotransferase HAMAP MF_00344
Ligand	ATP-binding HAMAP MF_00344 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00344 EMBL ABD10029.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	42 – 232	191	Glutamine amidotransferase type-1 By similarity HAMAP MF_00344
	Domain	264 – 431	168	GMP-binding By similarity HAMAP MF_00344
	Nucleotide binding	260 – 266	7	ATP By similarity HAMAP MF_00344
Sites
	Active site	119	1	Nucleophile By similarity HAMAP MF_00344
	Active site	206	1	By similarity HAMAP MF_00344
	Active site	208	1	By similarity HAMAP MF_00344

Sequences

	Sequence		Length	Mass (Da)
	Q2JFB3 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 0C1ACA5516F2DD7F	FASTA	556	59,886
	10 20 30 40 50 60 MQRSSAGRGA EPTDTGAVRD VPRSPADGPS GPSAVGPAGY DTVLVIDFGA QYAQLIARRI 70 80 90 100 110 120 RECHVYSEIV PWDMSVDEIL ARRPSALILS GGPKSVYSPG APRVDPALFA TGVPILGICY 130 140 150 160 170 180 GHQVMAQALD GAVARTGTAE YGATRLSVSD PGVLFDGLPV VQQVWMSHGD SVTAAPPGFR 190 200 210 220 230 240 VTASTPSAPV AAFEDPARRL YGVQFHPEVV HSERGMDVLR HFLLVGAGCR PTWTMINIVD 250 260 270 280 290 300 EAVAAVRAQV GTGRLICGLS GGVDSAVAAA LVHRAVGDAL TCVFVDHGLL RAGEAEQVER 310 320 330 340 350 360 DFVATTGVDL VHVKAADRFS AALSGVTDPE RKRKIIGREF IRVFEESARE LEVRAEAEGA 370 380 390 400 410 420 TIDFLVQGTL YPDVIESGSP TAAKIKSHHN VGGLPEDLRF DLVEPLRTLF KDEVRRLGEE 430 440 450 460 470 480 LGLPEEIVWR QPFPGPGLAV RIIGEVTPER LDVVRAADAV IREEIRKAGL DREIWQVFAV 490 500 510 520 530 540 LLADVRSVGV QGDERTYGFP IVLRAVTSED AMTADWARLP YGLLERISNR VVNEVPQVNR 550 VVYDITSKPP GTIEWE

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000249 Genomic DNA. Translation: ABD10029.1.
RefSeq	YP_479758.1. NC_007777.1.
3D structure databases
ProteinModelPortal	Q2JFB3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2JFB3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3903323.
GenomeReviews	Gene locus Francci3_0645 in contig CP000249_GR.
KEGG	fra:Francci3_0645.
PATRIC	21924166. VBIFraSp10456_0708.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0519.
HOGENOM	HBG391775.
OMA	DGRTYEY.
PhylomeDB	Q2JFB3.
ProtClustDB	PRK00074.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q2JFB3_FRASC

Accession

Primary (citable) accession number: Q2JFB3

Entry history

Integrated into UniProtKB/TrEMBL:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)