Reviewed, UniProtKB/Swiss-Prot Q2JES6 (URE1_FRASC)

Names and origin

Protein names

Recommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha

Gene names

Name:	ureC
Ordered Locus Names:	Francci3_0832

Organism

Frankia sp. (strain CcI3) [Complete proteome] [HAMAP]

Taxonomic identifier

106370 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity.
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW urease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Urease subunit alpha HAMAP MF_01953		PRO_0000239875
Regions
	Domain	138 – 575	438	Urease
Sites
	Active site	329	1	Proton donor By similarity
	Metal binding	143	1	Nickel 2 By similarity
	Metal binding	145	1	Nickel 2 By similarity
	Metal binding	226	1	Nickel 1; via carbamate group By similarity
	Metal binding	226	1	Nickel 2; via carbamate group By similarity
	Metal binding	255	1	Nickel 1 By similarity
	Metal binding	281	1	Nickel 1 By similarity
	Metal binding	369	1	Nickel 2 By similarity
	Binding site	228	1	Substrate By similarity
Amino acid modifications
	Modified residue	226	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2JES6-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 94389AA0821A0C59	FASTA	575	60,100
	10 20 30 40 50 60 MSRLDRSRYA ALYGPTVGDR IRLADTDLFI EVTEDRSRGP AGTGTGDEAV FGGGKVIRES 70 80 90 100 110 120 MGQSLATRAE GAPDLVITGA VVLDHWGVVK ADVGIRDGRI VALGKAGNPD TMDGVHPELV 130 140 150 160 170 180 MGPGTEIIAG NGKILTAGAV DCHVHLICPQ QVPEALGAGI TTLIGGGTGP AEGTKATTVT 190 200 210 220 230 240 PGSWNLARML SALDDWPVNV VLLGKGNTVS DESMWEQLRA GAAGFKLHED WGTTPAAIDA 250 260 270 280 290 300 CLRVADAAGV QVALHSDTLN EAGFVEDTLA AIAGRAIHAY HTEGAGGGHA PDIITVAAAG 310 320 330 340 350 360 NVLPSSTNPT RPHTVNTLDE HLDMLMVCHH LNPSVPEDLA FAESRIRPST IAAEDILHDL 370 380 390 400 410 420 GAISMIGSDS QAMGRIGEVV LRTWQTAHVM KLRRGSLAGD DRADNTRARR YIAKYTICPA 430 440 450 460 470 480 VAHGLDAEIG SVEPGKLADL VLYDPAFFGV RPSLVLKGGF IAWAAMGDAN ASIPTPQPVL 490 500 510 520 530 540 PRPMWGAARG PAAASSLIFV APAAIEDGLP GRLGLATPVV PVADVRRRGK ADLPENTATP 550 560 570 DIRVDPDTFT VTVDGEAIEA DPVSELPMTQ RYFLF

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000249 Genomic DNA. Translation: ABD10216.1.
RefSeq	YP_479945.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2JES6.
Genome annotation databases
GeneID	3905109.
GenomeReviews	Gene locus Francci3_0832 in contig CP000249_GR.
KEGG	fra:Francci3_0832.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2JES6.
OMA	IAGRAIH.
Enzyme and pathway databases
BioCyc	FSP106370:FRANCCI3_0832-MON.
Family and domain databases
HAMAP	MF_01953. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011612. Urease_alpha_N. IPR005848. Urease_asu. IPR017951. Urease_asu_c. IPR017952. Urease_asu_core. IPR017950. Urease_asu_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
PRINTS	PR01752. UREASE.
TIGRFAMs	TIGR01792. urease_alph. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. 1 hit. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URE1_FRASC

Accession

Primary (citable) accession number: Q2JES6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)