Q2JD58 (RSMH_FRASC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	Francci3_1407

Organism

Frankia sp. (strain CcI3) [Complete proteome] [HAMAP]

Taxonomic identifier

106370 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000386904
Regions
	Region	29 – 31	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	48	1	S-adenosyl-L-methionine By similarity
	Binding site	76	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	97	1	S-adenosyl-L-methionine By similarity
	Binding site	104	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2JD58 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: FA7B401921B81F5E	FASTA	321	34,548
	10 20 30 40 50 60 MLTDRVLTLL APALRASGAI VVDATVGLGG HAAAVLTAFP HVRLVGLDRD PDALERSGER 70 80 90 100 110 120 LHGLAPGRVE LVHAVYDEVA EILDRLGHPV VQGILFDLGV SSLQLDTDDR GFAYSRDAPL 130 140 150 160 170 180 DMRMDQTRGR TAADVLNTYD ADALTRILRE YGEERFARRI AGAIVQARAV TPFATSARLA 190 200 210 220 230 240 DLVRDAIPAP ARRTGGNPAK RTFQALRIEV NAELDVLARA LPAAFDALAV SGRLVVLSYH 250 260 270 280 290 300 SLEDRLVKRQ LRGYAEASVP PDLPVVPEGA GPRLRWLTRG AEPASETEKA ENPRAASVRL 310 320 RAVERTAPNP DHTRKPTGGA S

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CcI3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000249 Genomic DNA. Translation: ABD10784.1.
RefSeq	YP_480513.1. NC_007777.1.
3D structure databases
ProteinModelPortal	Q2JD58.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2JD58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3903388.
GenomeReviews	Gene locus Francci3_1407 in contig CP000249_GR.
KEGG	fra:Francci3_1407.
PATRIC	21925808. VBIFraSp10456_1519.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0275.
HOGENOM	HBG302779.
OMA	NPATRAF.
PhylomeDB	Q2JD58.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	FSP106370:FRANCCI3_1407-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_FRASC

Accession

Primary (citable) accession number: Q2JD58

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program