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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Frankia sp. (strain CcI3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67ZincUniRule annotation1
Binding sitei82Cysteinyl adenylateUniRule annotation1
Binding sitei247Cysteinyl adenylateUniRule annotation1
Metal bindingi251ZincUniRule annotation1
Metal bindingi276ZincUniRule annotation1
Binding sitei303Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:Francci3_2642
OrganismiFrankia sp. (strain CcI3)
Taxonomic identifieri106370 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000001937 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004004461 – 464L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseAdd BLAST464

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi106370.Francci3_2642.

Structurei

3D structure databases

ProteinModelPortaliQ2J9N8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 70Cysteinyl adenylate bindingUniRule annotation4
Regioni105 – 107Cysteinyl adenylate bindingUniRule annotation3
Regioni269 – 271Cysteinyl adenylate bindingUniRule annotation3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi69 – 79"HIGH" regionUniRule annotationAdd BLAST11
Motifi207 – 212"ERGGDP" regionUniRule annotation6
Motifi309 – 313"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiHIECATI.
OrthoDBiPOG091H004K.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2J9N8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSATLRLLS LCPPESRFAV TLTRMQAWPS PPIRSLPGHG KPLRIFDTAT
60 70 80 90 100
SSVRELAPAV TARLYVCGIT PYDATHLGHA FTYLTYDLAQ RVLRDAGHHV
110 120 130 140 150
HYVQNVTDVD DPLLERATRD GLDWRALADR EIDLFREDMT ALRMLAPDAY
160 170 180 190 200
VGVVEAIPMI VDMVVELVDR GAAYQVDDDL YFSIATAPAF GEISHLSRAE
210 220 230 240 250
MLAICAERGG DPRRTGKKDP LDPLLWRAHR PGEPSWPSPF GPGRPGWHIE
260 270 280 290 300
CSAIARHYLG GVIDIQGGGT DLSFPHHECS AAHAEVAAGI RPFARSYVHT
310 320 330 340 350
AMVSLDGHKM SKSRGNLEFV SRLRRAGVDP AALRLALLDH RHTEDWEWTP
360 370 380 390 400
GLLDDAVDRM NRWRAAVALP TGPDAMGLLA AVRERLADDL DAPGAVAAVD
410 420 430 440 450
AWVGAALADA GGSAGAGPDP THQGGPVRGS GGDVPAWGEA PALVRRLVDT
460
LLGVDLEPVR PRGS
Length:464
Mass (Da):50,147
Last modified:March 7, 2006 - v1
Checksum:iF534FFF62DDE7677
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD12004.1.
RefSeqiWP_011437039.1. NC_007777.1.

Genome annotation databases

EnsemblBacteriaiABD12004; ABD12004; Francci3_2642.
KEGGifra:Francci3_2642.
PATRICi21928522. VBIFraSp10456_2875.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD12004.1.
RefSeqiWP_011437039.1. NC_007777.1.

3D structure databases

ProteinModelPortaliQ2J9N8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi106370.Francci3_2642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD12004; ABD12004; Francci3_2642.
KEGGifra:Francci3_2642.
PATRICi21928522. VBIFraSp10456_2875.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiHIECATI.
OrthoDBiPOG091H004K.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHC_FRASC
AccessioniPrimary (citable) accession number: Q2J9N8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 7, 2006
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.