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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Frankia sp. (strain CcI3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671ZincUniRule annotation
Binding sitei82 – 821Cysteinyl adenylateUniRule annotation
Binding sitei247 – 2471Cysteinyl adenylateUniRule annotation
Metal bindingi251 – 2511ZincUniRule annotation
Metal bindingi276 – 2761ZincUniRule annotation
Binding sitei303 – 3031Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciFSP106370:GI1F-2676-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:Francci3_2642
OrganismiFrankia sp. (strain CcI3)
Taxonomic identifieri106370 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000001937 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligasePRO_0000400446Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi106370.Francci3_2642.

Structurei

3D structure databases

ProteinModelPortaliQ2J9N8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 704Cysteinyl adenylate bindingUniRule annotation
Regioni105 – 1073Cysteinyl adenylate bindingUniRule annotation
Regioni269 – 2713Cysteinyl adenylate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 7911"HIGH" regionUniRule annotationAdd
BLAST
Motifi207 – 2126"ERGGDP" regionUniRule annotation
Motifi309 – 3135"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiHANTYVS.
OrthoDBiEOG6RVFXC.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2J9N8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSATLRLLS LCPPESRFAV TLTRMQAWPS PPIRSLPGHG KPLRIFDTAT
60 70 80 90 100
SSVRELAPAV TARLYVCGIT PYDATHLGHA FTYLTYDLAQ RVLRDAGHHV
110 120 130 140 150
HYVQNVTDVD DPLLERATRD GLDWRALADR EIDLFREDMT ALRMLAPDAY
160 170 180 190 200
VGVVEAIPMI VDMVVELVDR GAAYQVDDDL YFSIATAPAF GEISHLSRAE
210 220 230 240 250
MLAICAERGG DPRRTGKKDP LDPLLWRAHR PGEPSWPSPF GPGRPGWHIE
260 270 280 290 300
CSAIARHYLG GVIDIQGGGT DLSFPHHECS AAHAEVAAGI RPFARSYVHT
310 320 330 340 350
AMVSLDGHKM SKSRGNLEFV SRLRRAGVDP AALRLALLDH RHTEDWEWTP
360 370 380 390 400
GLLDDAVDRM NRWRAAVALP TGPDAMGLLA AVRERLADDL DAPGAVAAVD
410 420 430 440 450
AWVGAALADA GGSAGAGPDP THQGGPVRGS GGDVPAWGEA PALVRRLVDT
460
LLGVDLEPVR PRGS
Length:464
Mass (Da):50,147
Last modified:March 7, 2006 - v1
Checksum:iF534FFF62DDE7677
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD12004.1.
RefSeqiWP_011437039.1. NC_007777.1.

Genome annotation databases

EnsemblBacteriaiABD12004; ABD12004; Francci3_2642.
KEGGifra:Francci3_2642.
PATRICi21928522. VBIFraSp10456_2875.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD12004.1.
RefSeqiWP_011437039.1. NC_007777.1.

3D structure databases

ProteinModelPortaliQ2J9N8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi106370.Francci3_2642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD12004; ABD12004; Francci3_2642.
KEGGifra:Francci3_2642.
PATRICi21928522. VBIFraSp10456_2875.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiHANTYVS.
OrthoDBiEOG6RVFXC.

Enzyme and pathway databases

BioCyciFSP106370:GI1F-2676-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CcI3.

Entry informationi

Entry nameiMSHC_FRASC
AccessioniPrimary (citable) accession number: Q2J9N8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 7, 2006
Last modified: January 20, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.