Reviewed, UniProtKB/Swiss-Prot Q2J8P2 (METK1_FRASC)

Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase 1
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 1
    AdoMet synthetase 1
    MAT 1

Gene names

Name:	metK1
Ordered Locus Names:	Francci3_2993

Organism

Frankia sp. (strain CcI3) [Complete proteome] [HAMAP]

Taxonomic identifier

106370 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. HAMAP MF_00086
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. HAMAP MF_00086
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00086
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP methionine adenosyltransferase activity Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	S-adenosylmethionine synthetase 1 HAMAP MF_00086		PRO_0000240995
Regions
	Nucleotide binding	276 – 283	8	ATP Potential
Sites
	Metal binding	17	1	Magnesium By similarity
	Metal binding	43	1	Potassium By similarity
	Metal binding	280	1	Potassium By similarity
	Metal binding	288	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2J8P2-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 8C5DFFC3850DEFB7	FASTA	401	42,893
	10 20 30 40 50 60 MSRRLFTSES VTEGHPDKLA DQISDALLDA FLAGDPTSRV AVETLLTTGH VHVAGEVTSS 70 80 90 100 110 120 AHVDVTAVVR RTLTDIGYDA ATGFDGATVG VLVSIGAQSP DIAQGVDKAY EARVEGGEDT 130 140 150 160 170 180 LAQQGAGDQG IMFGYATDET PDLLPLPITL AHRLAQRLSK VRHDGLLPYL RPDGKTQVTI 190 200 210 220 230 240 EYDGDRPVRL DTVVVSAHHA ADVDLATRLV PDIRDQVIAP ELKRLAEENL GLEVDGYRLL 250 260 270 280 290 300 VNPTGRFEIG GPIGDAGLTG RKIIIDTYGG YARHGGGAFS GKDPSKVDRS AAYALRWVAK 310 320 330 340 350 360 NVVAAGLARR AELQVAYAIG KAEPVGLFVE TFGTETVPVP EIARAVSEVF DLRPAAIIRD 370 380 390 400 LDLLRPIYGQ TASYGHFGRT LPDFTWERTD RADALRAAVG R

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000249 Genomic DNA. Translation: ABD12350.1.
RefSeq	YP_482079.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2J8P2.
Genome annotation databases
GeneID	3905490.
GenomeReviews	Gene locus Francci3_2993 in contig CP000249_GR.
KEGG	fra:Francci3_2993.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2J8P2.
OMA	DQVIAPE.
Enzyme and pathway databases
BioCyc	FSP106370:FRANCCI3_2993-MON.
Family and domain databases
HAMAP	MF_00086. [Tree]
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK1_FRASC

Accession

Primary (citable) accession number: Q2J8P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)