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Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene

pfp

Organism
Frankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.UniRule annotation

Catalytic activityi

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Non-allosteric.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10Diphosphate; via amide nitrogenUniRule annotation1
Metal bindingi103Magnesium; catalyticUniRule annotation1
Sitei104Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATPUniRule annotation1
Sitei124Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPiUniRule annotation1
Active sitei127Proton acceptorUniRule annotation1
Binding sitei162Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei221SubstrateUniRule annotation1
Binding sitei265Substrate; shared with dimeric partnerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationSAAS annotationImported, Transferase
Biological processGlycolysisUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciFSP106370:GI1F-3084-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotation (EC:2.7.1.90UniRule annotation)
Alternative name(s):
6-phosphofructokinase, pyrophosphate dependentUniRule annotation
PPi-dependent phosphofructokinaseUniRule annotation
Short name:
PPi-PFKUniRule annotation
Pyrophosphate-dependent 6-phosphofructose-1-kinaseUniRule annotation
Gene namesi
Name:pfpUniRule annotation
Ordered Locus Names:Francci3_3085Imported
OrganismiFrankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3)Imported
Taxonomic identifieri106370 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000001937 Componenti: Chromosome

Subcellular locationi

Q2J8F0:
  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi106370.Francci3_3085.

Structurei

3D structure databases

ProteinModelPortaliQ2J8F0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 296PFKInterPro annotationAdd BLAST295

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 127Substrate bindingUniRule annotation3
Regioni169 – 171Substrate bindingUniRule annotation3
Regioni271 – 274Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
KOiK21071.
OMAiRDGWRGP.
OrthoDBiPOG091H01AC.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiView protein in InterPro
IPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
IPR035966. PKF_sf.
PfamiView protein in Pfam
PF00365. PFK. 1 hit.
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiView protein in PROSITE
PS00433. PHOSPHOFRUCTOKINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2J8F0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGVLTGGG DCPGLNAVIR AVVRKGESVY GHSFVGYRDG WRGPLTGETV
60 70 80 90 100
PLDIAAVRGI LPRGGTILGS SRTNPYAAVD GPALVREHLA AAEVDALIAI
110 120 130 140 150
GGEDTLGVAA QLHAEGLPVV GVPKTIDNDL SATDYTFGFD TAVNIATEAI
160 170 180 190 200
DRLHTTAESH HRVLIVEVMG RHAGWIALHS GMAGGANVIL IPERPFDFDK
210 220 230 240 250
VCAFVEARFA THYAPIIVAA EGAVPITGTL VAREGEVDAF GHVRLQGIGA
260 270 280 290 300
VLETEIRART GRDARATVLG HLQRGGTPTA FDRWLATRFG LHAVDAVHEG
310 320 330 340
DFGVMVALHG TRIDRVPLQD ATRELKTVPE ELYHEAEIFF G
Length:341
Mass (Da):36,188
Last modified:March 7, 2006 - v1
Checksum:i4CD35870930C439F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD12442.1.
RefSeqiWP_011437470.1. NC_007777.1.

Genome annotation databases

EnsemblBacteriaiABD12442; ABD12442; Francci3_3085.
GeneIDi32157948.
KEGGifra:Francci3_3085.

Similar proteinsi

Entry informationi

Entry nameiQ2J8F0_FRACC
AccessioniPrimary (citable) accession number: Q2J8F0
Entry historyiIntegrated into UniProtKB/TrEMBL: March 7, 2006
Last sequence update: March 7, 2006
Last modified: October 25, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported