Q2J858 (Q2J858_FRASC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281
EC=6.1.1.20 HAMAP MF_00281

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281

Gene names

Name:	pheS HAMAP MF_00281
Ordered Locus Names:	Francci3_3177

Organism

Frankia sp. (strain CcI3) [Complete proteome] [HAMAP] EMBL ABD12534.1

Taxonomic identifier

106370 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity. HAMAP MF_00281
Subcellular location	Cytoplasm By similarity HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily. HAMAP MF_00281

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00281
Cellular component	Cytoplasm HAMAP MF_00281
Ligand	ATP-binding HAMAP MF_00281 Magnesium HAMAP MF_00281 Metal-binding HAMAP MF_00281 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00281 EMBL ABD12534.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q2J858 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 9ACAA2D8F66C685B	FASTA	367	39,959
	10 20 30 40 50 60 MPAPGETVDP SRVHALDPDQ LDAAVNAARE SIRAAADLDA LAAVRTAHIQ GASAPLVLAR 70 80 90 100 110 120 RELGALPRAE RADAGRRHNA ARTAVQAAYD ARLAELTAER DARVLREERV DVTLPVDRRR 130 140 150 160 170 180 RGARHPLTAL SDRLADVFVA MGYEVAEGPE VEHDWFNFEA LNMAPDHPAR SEHDTLYVAP 190 200 210 220 230 240 TGAGRVLRTH TSPVQIRSLL NRPLPVYVVC PGRTYRNDTI DATHSPVFGQ LEGLAVDRGI 250 260 270 280 290 300 TMADLRGTLE VFAQALFGAG LSTRLRPSFF PFTEPSAELD VQCFACRGEA ARQPCRVCSD 310 320 330 340 350 360 EGWIEAGGCG MVDPHVLRAA GVDPTRYSGF AFGMGLERAA MVRHGIREIR DFVDGDVRFS LPFGIED

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000249 Genomic DNA. Translation: ABD12534.1.
RefSeq	YP_482263.1. NC_007777.1.
3D structure databases
ProteinModelPortal	Q2J858.
SMR	Q2J858. Positions 99-363.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2J858.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3903902.
GenomeReviews	Gene locus Francci3_3177 in contig CP000249_GR.
KEGG	fra:Francci3_3177.
PATRIC	21929674. VBIFraSp10456_3449.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	Q2J858.
ProtClustDB	PRK00488.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q2J858_FRASC

Accession

Primary (citable) accession number: Q2J858

Entry history

Integrated into UniProtKB/TrEMBL:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)