Q2J850 (RISB_FRASC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:	ribH
Ordered Locus Names:	Francci3_3185

Organism

Frankia sp. (strain CcI3) [Complete proteome] [HAMAP]

Taxonomic identifier

106370 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 160	160	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_1000040422

Sequences

	Sequence		Length	Mass (Da)
	Q2J850 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 79A0F00A06714043	FASTA	160	16,138
	10 20 30 40 50 60 MSGLGAPAEV LPPVAGMRLA VVATRWHAEI TDALLAGALR AAKDAGLAAA PAVVRVSGAV 70 80 90 100 110 120 ELPVIAAALA ARHDAVVALG VVIRGGTPHF DYVCQFVTAG LARVTLDARV PVGFGVLTCD 130 140 150 160 TVEQARDRAG LPGSSEDKGR EATLAALDTA AVLRDLELAG

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CcI3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000249 Genomic DNA. Translation: ABD12542.1.
RefSeq	YP_482271.1. NC_007777.1.
3D structure databases
ProteinModelPortal	Q2J850.
SMR	Q2J850. Positions 15-156.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2J850.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3903910.
GenomeReviews	Gene locus Francci3_3185 in contig CP000249_GR.
KEGG	fra:Francci3_3185.
PATRIC	21929690. VBIFraSp10456_3457.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0054.
HOGENOM	HBG311126.
OMA	SSRALMD.
PhylomeDB	Q2J850.
ProtClustDB	PRK00061.
Enzyme and pathway databases
BioCyc	FSP106370:FRANCCI3_3185-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_FRASC

Accession

Primary (citable) accession number: Q2J850

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program