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Protein

6,7-dimethyl-8-ribityllumazine synthase

Gene

ribH

Organism
Frankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.UniRule annotation

Catalytic activityi

1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate.UniRule annotation

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 6,7-dimethyl-8-ribityllumazine synthase (ribH)
  2. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265-amino-6-(D-ribitylamino)uracilUniRule annotation1
Active sitei89Proton donorUniRule annotation1
Binding sitei1145-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei1281-deoxy-L-glycero-tetrulose 4-phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processRiboflavin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00275; UER00404

Names & Taxonomyi

Protein namesi
Recommended name:
6,7-dimethyl-8-ribityllumazine synthaseUniRule annotation (EC:2.5.1.78UniRule annotation)
Short name:
DMRL synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lumazine synthaseUniRule annotation
Gene namesi
Name:ribHUniRule annotation
Ordered Locus Names:Francci3_3185
OrganismiFrankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3)
Taxonomic identifieri106370 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000001937 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000404221 – 1606,7-dimethyl-8-ribityllumazine synthaseAdd BLAST160

Interactioni

Protein-protein interaction databases

STRINGi106370.Francci3_3185

Structurei

3D structure databases

ProteinModelPortaliQ2J850
SMRiQ2J850
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 615-amino-6-(D-ribitylamino)uracil bindingUniRule annotation3
Regioni81 – 835-amino-6-(D-ribitylamino)uracil bindingUniRule annotation3
Regioni86 – 871-deoxy-L-glycero-tetrulose 4-phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DMRL synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UTT Bacteria
COG0054 LUCA
HOGENOMiHOG000229253
KOiK00794
OMAiHGNKGTE
OrthoDBiPOG091H021R

Family and domain databases

CDDicd09209 Lumazine_synthase-I, 1 hit
Gene3Di3.40.50.960, 1 hit
HAMAPiMF_00178 Lumazine_synth, 1 hit
InterProiView protein in InterPro
IPR034964 LS
IPR002180 LS/RS
IPR036467 LS/RS_sf
PANTHERiPTHR21058 PTHR21058, 1 hit
PfamiView protein in Pfam
PF00885 DMRL_synthase, 1 hit
SUPFAMiSSF52121 SSF52121, 1 hit
TIGRFAMsiTIGR00114 lumazine-synth, 1 hit

Sequencei

Sequence statusi: Complete.

Q2J850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLGAPAEV LPPVAGMRLA VVATRWHAEI TDALLAGALR AAKDAGLAAA
60 70 80 90 100
PAVVRVSGAV ELPVIAAALA ARHDAVVALG VVIRGGTPHF DYVCQFVTAG
110 120 130 140 150
LARVTLDARV PVGFGVLTCD TVEQARDRAG LPGSSEDKGR EATLAALDTA
160
AVLRDLELAG
Length:160
Mass (Da):16,138
Last modified:March 7, 2006 - v1
Checksum:i79A0F00A06714043
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA Translation: ABD12542.1
RefSeqiWP_011437570.1, NC_007777.1

Genome annotation databases

EnsemblBacteriaiABD12542; ABD12542; Francci3_3185
GeneIDi32158047
KEGGifra:Francci3_3185

Similar proteinsi

Entry informationi

Entry nameiRISB_FRACC
AccessioniPrimary (citable) accession number: Q2J850
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: March 28, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health