Reviewed, UniProtKB/Swiss-Prot Q2J843 (METK2_FRASC)

Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase 2
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 2
    AdoMet synthetase 2
    MAT 2

Gene names

Name:	metK2
Ordered Locus Names:	Francci3_3192

Organism

Frankia sp. (strain CcI3) [Complete proteome] [HAMAP]

Taxonomic identifier

106370 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity. HAMAP MF_00086
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. HAMAP MF_00086
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. HAMAP MF_00086 Binds 1 potassium ion per subunit By similarity. HAMAP MF_00086
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. HAMAP MF_00086
Subunit structure	Homotetramer By similarity. HAMAP MF_00086
Subcellular location	Cytoplasm HAMAP MF_00086.
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP methionine adenosyltransferase activity Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	S-adenosylmethionine synthetase 2 HAMAP MF_00086		PRO_0000240996
Regions
	Nucleotide binding	273 – 280	8	ATP Potential
Sites
	Metal binding	17	1	Magnesium By similarity
	Metal binding	43	1	Potassium By similarity
	Metal binding	277	1	Potassium By similarity
	Metal binding	285	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2J843-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 1A3185E5393795B9	FASTA	398	42,564
	10 20 30 40 50 60 MATRLFTSES VTEGHPDKIA DQISDSILDA MIKDDPKSRV AVETLITTGQ VHVAGEVTTK 70 80 90 100 110 120 TYVDIASVVR ERILEIGYDS SKKGFDGASC GVSVSIGAQS PDIAQGVDTA HETRVEGSAD 130 140 150 160 170 180 DEFDRQGAGD QGLMFGFACD ETPELMPLPI ALAHRLARRL SAVRKEGQIG YLRPDGKTQV 190 200 210 220 230 240 TIEYVDGKPA RLDTVVVSSQ HAADIDLDTL LAPDIAEYVV EPELALLEIA TEGRRLLVNP 250 260 270 280 290 300 TGRFEIGGPM GDAGLTGRKI IVDTYGGYAR HGGGAFSGKD PSKVDRSAAY AMRWVAKNVV 310 320 330 340 350 360 AAGLASRCEV QVAYAIGKAQ PVGLFVETFG TGTVPDIQIQ DAVTSVFDLR PAAIIRDLDL 370 380 390 LRPVYAQTAA YGHFGRPELD FTWESTARAE ALQRAVKG

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000249 Genomic DNA. Translation: ABD12549.1.
RefSeq	YP_482278.1.
3D structure databases
SMR	Q2J843. Positions 3-397.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2J843.
Genome annotation databases
GeneID	3903918.
GenomeReviews	Gene locus Francci3_3192 in contig CP000249_GR.
KEGG	fra:Francci3_3192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0192.
HOGENOM	HBG443662.
OMA	IYYAHRL.
PhylomeDB	Q2J843.
Family and domain databases
HAMAP	MF_00086. S-AdoMet_synth1. [Tree]
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK2_FRASC

Accession

Primary (citable) accession number: Q2J843

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)