Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)

Gene

ispG

Organism
Frankia sp. (strain CcI3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.UniRule annotation

Catalytic activityi

(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 5 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi280Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi283Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi315Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi322Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00056; UER00096.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)UniRule annotation (EC:1.17.7.3UniRule annotation)
Alternative name(s):
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthaseUniRule annotation
Gene namesi
Name:ispGUniRule annotation
Ordered Locus Names:Francci3_3573
OrganismiFrankia sp. (strain CcI3)
Taxonomic identifieri106370 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000001937 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001234481 – 3844-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)Add BLAST384

Interactioni

Protein-protein interaction databases

STRINGi106370.Francci3_3573.

Structurei

3D structure databases

ProteinModelPortaliQ2J717.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4E. Bacteria.
COG0821. LUCA.
HOGENOMiHOG000261019.
KOiK03526.
OMAiHNGMKIA.
OrthoDBiPOG091H011D.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00159. IspG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011005. Dihydropteroate_synth-like.
IPR016425. IspG_bac.
IPR004588. IspG_bac-typ.
[Graphical view]
PfamiPF04551. GcpE. 1 hit.
[Graphical view]
PIRSFiPIRSF004640. IspG. 1 hit.
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR00612. ispG_gcpE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2J717-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTLGMPTA PARPLGTRRH SRQIHVGNVL VGGDAPVSVQ SMCTTLTSDV
60 70 80 90 100
NATLQQIAQL TASGCQIVRV AVPSQDDADA LAAIARKSPI PVIADIHFQP
110 120 130 140 150
KYVFAAIDAG CAAVRVNPGN IKAFDDKVGE IARAAKAAGV PIRIGVNAGS
160 170 180 190 200
LDKRLLAKYG KATPEALTES ALWECSLFEE HDFRDIKISV KHHDPVVMIQ
210 220 230 240 250
AYRLLAQACD YPLHLGVTEA GPSFQGTVKS AVAFGALLAE GIGDTIRVSL
260 270 280 290 300
SAPPVEEVKV GTAILESLGL RQRKLEIVSC PSCGRAQVDV YTLANQVSAG
310 320 330 340 350
LEGMEVPLRV AVMGCVVNGP GEAREADLGV ASGNGKGQIF VRGEVVKTVP
360 370 380
EAQIVETLIE EAMRLAEEMA ADGTPSGEPS VSVG
Length:384
Mass (Da):40,140
Last modified:March 7, 2006 - v1
Checksum:iFE5BC70132E2E378
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD12925.1.
RefSeqiWP_011437949.1. NC_007777.1.

Genome annotation databases

EnsemblBacteriaiABD12925; ABD12925; Francci3_3573.
KEGGifra:Francci3_3573.
PATRICi21930574. VBIFraSp10456_3897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD12925.1.
RefSeqiWP_011437949.1. NC_007777.1.

3D structure databases

ProteinModelPortaliQ2J717.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi106370.Francci3_3573.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD12925; ABD12925; Francci3_3573.
KEGGifra:Francci3_3573.
PATRICi21930574. VBIFraSp10456_3897.

Phylogenomic databases

eggNOGiENOG4105C4E. Bacteria.
COG0821. LUCA.
HOGENOMiHOG000261019.
KOiK03526.
OMAiHNGMKIA.
OrthoDBiPOG091H011D.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00096.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00159. IspG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011005. Dihydropteroate_synth-like.
IPR016425. IspG_bac.
IPR004588. IspG_bac-typ.
[Graphical view]
PfamiPF04551. GcpE. 1 hit.
[Graphical view]
PIRSFiPIRSF004640. IspG. 1 hit.
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR00612. ispG_gcpE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiISPG_FRASC
AccessioniPrimary (citable) accession number: Q2J717
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 7, 2006
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.