Q2J6Z9 (TRMD_FRASC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Francci3_3591

Organism

Frankia sp. (strain CcI3) [Complete proteome] [HAMAP]

Taxonomic identifier

106370 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000257422
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	113	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2J6Z9 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: B3027A8002999DDD	FASTA	270	29,535
	10 20 30 40 50 60 MRADIVTIFP AYLEPLRLSL VGRAQEHGTL QIHTHDLRMW TTDVHRTVDD APYGGGPGMV 70 80 90 100 110 120 MRPEPWDAAL SQITARVADS RPRVVVPTPA GTPFTQRHAE ELAREPWLVF CCGRYEGIDA 130 140 150 160 170 180 RVIETWADDE ISIGDYVLAG GEAATLVILE AVTRLLPGVL GNSASVADDS FSDGLLEGPV 190 200 210 220 230 240 YTRPPVWRGT EVPPVLRSGD HEAIARWRRS QALRRTLLRR PDLLEHREFA EADRVVLAAA 250 260 270 AAEAAGRMLP APTEGTGLIH HRDVEGPGEG

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CcI3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000249 Genomic DNA. Translation: ABD12943.1.
RefSeq	YP_482672.1. NC_007777.1.
3D structure databases
ProteinModelPortal	Q2J6Z9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2J6Z9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3904145.
GenomeReviews	Gene locus Francci3_3591 in contig CP000249_GR.
KEGG	fra:Francci3_3591.
PATRIC	21930614. VBIFraSp10456_3917.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	VCGRFEG.
PhylomeDB	Q2J6Z9.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	FSP106370:FRANCCI3_3591-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_FRASC

Accession

Primary (citable) accession number: Q2J6Z9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program