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Protein

Ketol-acid reductoisomerase (NADP(+))

Gene

ilvC

Organism
Frankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.UniRule annotation

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.UniRule annotation
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (Francci3_3640)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (Francci3_3640)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51NADPUniRule annotation1
Binding sitei53NADPUniRule annotation1
Active sitei108UniRule annotation1
Binding sitei134NADP; via amide nitrogenUniRule annotation1
Metal bindingi191Magnesium 1UniRule annotation1
Metal bindingi191Magnesium 2UniRule annotation1
Metal bindingi195Magnesium 1UniRule annotation1
Metal bindingi227Magnesium 2UniRule annotation1
Metal bindingi231Magnesium 2UniRule annotation1
Binding sitei252SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 28NADPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandMagnesium, Metal-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00047; UER00056.
UPA00049; UER00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase (NADP(+))UniRule annotation (EC:1.1.1.86UniRule annotation)
Short name:
KARIUniRule annotation
Alternative name(s):
Acetohydroxy-acid isomeroreductaseUniRule annotation
Short name:
AHIRUniRule annotation
Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation
Ketol-acid reductoisomerase type 1UniRule annotation
Ketol-acid reductoisomerase type IUniRule annotation
Gene namesi
Name:ilvCUniRule annotation
Ordered Locus Names:Francci3_3638
OrganismiFrankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3)
Taxonomic identifieri106370 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000001937 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002527601 – 329Ketol-acid reductoisomerase (NADP(+))Add BLAST329

Interactioni

Protein-protein interaction databases

STRINGi106370.Francci3_3638.

Structurei

3D structure databases

ProteinModelPortaliQ2J6V2.
SMRiQ2J6V2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 178IlvNUniRule annotationAdd BLAST164
Domaini184 – 327IlvCUniRule annotationAdd BLAST144

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6M. Bacteria.
COG0059. LUCA.
HOGENOMiHOG000016230.
KOiK00053.
OMAiRAMFSWL.
OrthoDBiPOG091H063Y.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.50.50.60. 1 hit.
HAMAPiMF_00435. IlvC. 1 hit.
InterProiView protein in InterPro
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR000506. AcH_isomrdctse_C.
IPR023753. FAD/NAD-binding_dom.
IPR013116. IlvN.
IPR013023. KARI.
IPR014359. KARI_prok.
IPR016040. NAD(P)-bd_dom.
PANTHERiPTHR21371. PTHR21371. 1 hit.
PfamiView protein in Pfam
PF01450. IlvC. 1 hit.
PF07991. IlvN. 1 hit.
PIRSFiPIRSF000116. IlvC_gammaproteo. 2 hits.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00465. ilvC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2J6V2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEIYYDDDA SLDVLADRKV AVIGYGSQGH AHALNLRDSG VDVRVGLPAD
60 70 80 90 100
SRSRARAEEE GLRVLTPAEA SAEADIIMLL TPDTTHRTIY AESIAPHLTA
110 120 130 140 150
GKALAFGHGF NIRYGLIEPP AGVDVFMVAP KGPGHLVRRV FEEGKGVPVL
160 170 180 190 200
VAVEADASGN ALAVALAYAK GIGGTRAGAL RTTFTEETET DLFGEQAVLC
210 220 230 240 250
GGASALVQAG FETLVEAGYT PEVAYFECLH ELKLIVDLMY EGGISQMRYS
260 270 280 290 300
ISDTAEYGDV TRGPRVITPA VKAEMRKILD EIRDGAFARE WVAEDDNGRP
310 320
NFTKLVAEGK QHPIEQVGAK LRPMMSWIA
Length:329
Mass (Da):35,342
Last modified:March 7, 2006 - v1
Checksum:i2632C3400B2E7A18
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD12990.1.
RefSeqiWP_011438014.1. NC_007777.1.

Genome annotation databases

EnsemblBacteriaiABD12990; ABD12990; Francci3_3638.
GeneIDi32158486.
KEGGifra:Francci3_3638.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiILVC_FRACC
AccessioniPrimary (citable) accession number: Q2J6V2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: March 7, 2006
Last modified: July 5, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families