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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Frankia sp. (strain CcI3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei490 – 4901NucleophileUniRule annotation
Active sitei543 – 5431Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciFSP106370:GI1F-3729-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Francci3_3681
OrganismiFrankia sp. (strain CcI3)
Taxonomic identifieri106370 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia
ProteomesiUP000001937: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8128121,4-alpha-glucan branching enzyme GlgBPRO_0000260658Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi106370.Francci3_3681.

Structurei

3D structure databases

ProteinModelPortaliQ2J6Q9.
SMRiQ2J6Q9. Positions 201-811.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2J6Q9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNGDVNNGT ARTGRLATGL APDRPNPRGA ATAVRNGGGR VALADRDASS
60 70 80 90 100
ASAQPGQTAD DPAVPSAPPS APPSALPADP PAVAPAVSVD LPYDDLERLV
110 120 130 140 150
SGAHHDPHAL LGAHPHPGSD ATVVRVLRPD ARAVTVLVGP ARYPATRLHS
160 170 180 190 200
GGVFGVAVPG MLPDYRIEVT YPDGPYLIDD PYRHLPTLGE MDLHLIIEGR
210 220 230 240 250
HEQLWKVLGA HPRALTTPGG ATVTGVSFAV WAPSARGVRL VGDFDFWDGR
260 270 280 290 300
AFPMRSLGRS GIWELFVPGA GTGARYKYEI LGFDGIWRQK ADPLAFHTEV
310 320 330 340 350
PPATASVVFA SDFTWNDGAW LDRRARTAWR TEPVSVYEVH LGSWRRGLSY
360 370 380 390 400
RELAEELTAY VVENGFTHVE MLPVAEHPFG GSWGYQVSAY YAPTARFGSP
410 420 430 440 450
DEFRHLVDTL HRAGIGVIVD WVPAHFPRDT WALGRFDGTP LYEHPDPRRG
460 470 480 490 500
EQPDWGTYVF DLGRPEVRNF LVANALYWFE EFHIDGLRVD AVASMLYLDY
510 520 530 540 550
SRPEGGWLPN IHGGRENLDA VSFLQETNAT VYRRFPGAMM IAEESTAWPG
560 570 580 590 600
VTRPTHLGGL GFGFKWNMGW MHDTLDYNSR LPIHRMYHHH QMTFSMVYAY
610 620 630 640 650
SENFILPFSH DEVVHGKGSL LRKMPGDRWA QLANLRALLA YMWAHPGKKL
660 670 680 690 700
LFMGCEFAQD NEWNESASLE WPLLDDPAHA GVADLVRDLN GLYRTVPALY
710 720 730 740 750
QHDADPAGFS WIDANDAENN VFSFLRWSGE DPAGGVLACV TNFAGIGHEG
760 770 780 790 800
YRIGLPFPGR WREILNTDGY RYGGGNIGNL GSVQAVEEPH HGLDASATLT
810
LPPLGAIWLS PA
Length:812
Mass (Da):89,229
Last modified:March 7, 2006 - v1
Checksum:i7DA0746ADF835D01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD13033.1.
RefSeqiYP_482762.1. NC_007777.1.

Genome annotation databases

EnsemblBacteriaiABD13033; ABD13033; Francci3_3681.
GeneIDi3905365.
KEGGifra:Francci3_3681.
PATRICi21930812. VBIFraSp10456_4011.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD13033.1.
RefSeqiYP_482762.1. NC_007777.1.

3D structure databases

ProteinModelPortaliQ2J6Q9.
SMRiQ2J6Q9. Positions 201-811.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi106370.Francci3_3681.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD13033; ABD13033; Francci3_3681.
GeneIDi3905365.
KEGGifra:Francci3_3681.
PATRICi21930812. VBIFraSp10456_4011.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciFSP106370:GI1F-3729-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CcI3.

Entry informationi

Entry nameiGLGB_FRASC
AccessioniPrimary (citable) accession number: Q2J6Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: March 7, 2006
Last modified: January 7, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.