Reviewed, UniProtKB/Swiss-Prot Q2J6H9 (BIOD_FRASC)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	Francci3_3762

Organism

Frankia sp. (strain CcI3) [Complete proteome] [HAMAP]

Taxonomic identifier

106370 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 6-carboxyhexanoyl-CoA: step 3/4. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000302511
Regions
	Nucleotide binding	8 – 16	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2J6H9-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: E82AC3C553209B84	FASTA	238	24,273
	10 20 30 40 50 60 MTALVVTGTG TGVGKTVVTA AIGALAHDRH HAVAVVKPAQ TGVGPGELGD VDLVRDLTGI 70 80 90 100 110 120 TDVHELARYP DPLAPATAAR RSGHRAVDLD ELAARIGTLA DSRDLVLVEG AGGLLVRYDA 130 140 150 160 170 180 GGATLADLAR VLRAPVLVVT AAGLGALNDT ALTLEALAHR GLDLAGVVVG SWPAEPDLAC 190 200 210 220 230 RCNLIDLESL AARPLAGVLP AGAGLLARLE FLAVARASLQ PALGGTFRAQ EFRARYET

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000249 Genomic DNA. Translation: ABD13113.1.
RefSeq	YP_482842.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3906046.
GenomeReviews	Gene locus Francci3_3762 in contig CP000249_GR.
KEGG	fra:Francci3_3762.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2J6H9.
OMA	Q2J6H9. PAGIVFV.
Enzyme and pathway databases
BioCyc	FSP106370:FRANCCI3_3762-MON.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_FRASC

Accession

Primary (citable) accession number: Q2J6H9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)