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Q2J6H8 (BIOB1_FRASC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase 1

EC=2.8.1.6
Gene names
Name:bioB1
Ordered Locus Names:Francci3_3763
OrganismFrankia sp. (strain CcI3) [Complete proteome] [HAMAP]
Taxonomic identifier106370 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358Biotin synthase 1 HAMAP-Rule MF_01694
PRO_0000381388

Sites

Metal binding951Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding991Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1021Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1381Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1711Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2301Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding3001Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2J6H8 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: A83287834EF541A1

FASTA35838,232
        10         20         30         40         50         60 
MTASVTTSVT AHPTAPAPAS QRPPADQDGA DILTVARREV LDGGRGLDEA GVLAVLRLPD 

        70         80         90        100        110        120 
ETLTDLLALA HEVRMRWCGP EVEVEGIVSL KTGGCPEDCH FCSQSGKFDS PVRSAWLDVP 

       130        140        150        160        170        180 
SLVDAARQTA ATGATEFCIV AAVRGPDARL MAQVREGVAA IRAAVDINVA CSLGMLTSEQ 

       190        200        210        220        230        240 
VDELTAMGVH RYNHNLETAR SHFPNVVTTH SWEERWETCE MVRAAGMELC CGAILGVGES 

       250        260        270        280        290        300 
LEQRAELATQ LAALEPDEVP LNFLNPRPGT PFGDLPLVEP RDALRAIAAF RLAMPRTILR 

       310        320        330        340        350 
YSGGREITLG DLDVQGMLGG INAMIVGNYL TTLGRSAEAD LKMLAELSMP IKSLQATL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000249 Genomic DNA. Translation: ABD13114.1.
RefSeqYP_482843.1. NC_007777.1.

3D structure databases

ProteinModelPortalQ2J6H8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING106370.Francci3_3763.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD13114; ABD13114; Francci3_3763.
GeneID3906047.
KEGGfra:Francci3_3763.
PATRIC21930988. VBIFraSp10456_4098.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMAFADYEVL.
OrthoDBEOG622PMP.
ProtClustDBPRK06256.

Enzyme and pathway databases

BioCycFSP106370:GI1F-3813-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB1_FRASC
AccessionPrimary (citable) accession number: Q2J6H8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 7, 2006
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways