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Q2J6H8

- BIOB1_FRASC

UniProt

Q2J6H8 - BIOB1_FRASC

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Protein

Biotin synthase 1

Gene
bioB1, Francci3_3763
Organism
Frankia sp. (strain CcI3)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi102 – 1021Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi171 – 1711Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi230 – 2301Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi300 – 3001Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciFSP106370:GI1F-3813-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase 1 (EC:2.8.1.6)
Gene namesi
Name:bioB1
Ordered Locus Names:Francci3_3763
OrganismiFrankia sp. (strain CcI3)
Taxonomic identifieri106370 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia
ProteomesiUP000001937: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Biotin synthase 1UniRule annotationPRO_0000381388Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi106370.Francci3_3763.

Structurei

3D structure databases

ProteinModelPortaliQ2J6H8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiCIARITL.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2J6H8-1 [UniParc]FASTAAdd to Basket

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MTASVTTSVT AHPTAPAPAS QRPPADQDGA DILTVARREV LDGGRGLDEA    50
GVLAVLRLPD ETLTDLLALA HEVRMRWCGP EVEVEGIVSL KTGGCPEDCH 100
FCSQSGKFDS PVRSAWLDVP SLVDAARQTA ATGATEFCIV AAVRGPDARL 150
MAQVREGVAA IRAAVDINVA CSLGMLTSEQ VDELTAMGVH RYNHNLETAR 200
SHFPNVVTTH SWEERWETCE MVRAAGMELC CGAILGVGES LEQRAELATQ 250
LAALEPDEVP LNFLNPRPGT PFGDLPLVEP RDALRAIAAF RLAMPRTILR 300
YSGGREITLG DLDVQGMLGG INAMIVGNYL TTLGRSAEAD LKMLAELSMP 350
IKSLQATL 358
Length:358
Mass (Da):38,232
Last modified:March 7, 2006 - v1
Checksum:iA83287834EF541A1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000249 Genomic DNA. Translation: ABD13114.1.
RefSeqiYP_482843.1. NC_007777.1.

Genome annotation databases

EnsemblBacteriaiABD13114; ABD13114; Francci3_3763.
GeneIDi3906047.
KEGGifra:Francci3_3763.
PATRICi21930988. VBIFraSp10456_4098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000249 Genomic DNA. Translation: ABD13114.1 .
RefSeqi YP_482843.1. NC_007777.1.

3D structure databases

ProteinModelPortali Q2J6H8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 106370.Francci3_3763.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD13114 ; ABD13114 ; Francci3_3763 .
GeneIDi 3906047.
KEGGi fra:Francci3_3763.
PATRICi 21930988. VBIFraSp10456_4098.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi CIARITL.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci FSP106370:GI1F-3813-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CcI3.

Entry informationi

Entry nameiBIOB1_FRASC
AccessioniPrimary (citable) accession number: Q2J6H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 7, 2006
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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