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Q2J6H8

- BIOB1_FRASC

UniProt

Q2J6H8 - BIOB1_FRASC

Protein

Biotin synthase 1

Gene

bioB1

Organism
Frankia sp. (strain CcI3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (07 Mar 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi102 – 1021Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi171 – 1711Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi230 – 2301Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi300 – 3001Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciFSP106370:GI1F-3813-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthase 1UniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioB1UniRule annotation
    Ordered Locus Names:Francci3_3763
    OrganismiFrankia sp. (strain CcI3)
    Taxonomic identifieri106370 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia
    ProteomesiUP000001937: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Biotin synthase 1PRO_0000381388Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi106370.Francci3_3763.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2J6H8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiCIARITL.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2J6H8-1 [UniParc]FASTAAdd to Basket

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    MTASVTTSVT AHPTAPAPAS QRPPADQDGA DILTVARREV LDGGRGLDEA    50
    GVLAVLRLPD ETLTDLLALA HEVRMRWCGP EVEVEGIVSL KTGGCPEDCH 100
    FCSQSGKFDS PVRSAWLDVP SLVDAARQTA ATGATEFCIV AAVRGPDARL 150
    MAQVREGVAA IRAAVDINVA CSLGMLTSEQ VDELTAMGVH RYNHNLETAR 200
    SHFPNVVTTH SWEERWETCE MVRAAGMELC CGAILGVGES LEQRAELATQ 250
    LAALEPDEVP LNFLNPRPGT PFGDLPLVEP RDALRAIAAF RLAMPRTILR 300
    YSGGREITLG DLDVQGMLGG INAMIVGNYL TTLGRSAEAD LKMLAELSMP 350
    IKSLQATL 358
    Length:358
    Mass (Da):38,232
    Last modified:March 7, 2006 - v1
    Checksum:iA83287834EF541A1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000249 Genomic DNA. Translation: ABD13114.1.
    RefSeqiYP_482843.1. NC_007777.1.

    Genome annotation databases

    EnsemblBacteriaiABD13114; ABD13114; Francci3_3763.
    GeneIDi3906047.
    KEGGifra:Francci3_3763.
    PATRICi21930988. VBIFraSp10456_4098.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000249 Genomic DNA. Translation: ABD13114.1 .
    RefSeqi YP_482843.1. NC_007777.1.

    3D structure databases

    ProteinModelPortali Q2J6H8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 106370.Francci3_3763.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD13114 ; ABD13114 ; Francci3_3763 .
    GeneIDi 3906047.
    KEGGi fra:Francci3_3763.
    PATRICi 21930988. VBIFraSp10456_4098.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi CIARITL.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci FSP106370:GI1F-3813-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CcI3.

    Entry informationi

    Entry nameiBIOB1_FRASC
    AccessioniPrimary (citable) accession number: Q2J6H8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3