Q2J661 (Q2J661_FRASC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2 HAMAP MF_00191
EC=1.17.1.2 HAMAP MF_00191

Gene names

Name:	ispH2 HAMAP MF_00191
Ordered Locus Names:	Francci3_3881

Organism

Frankia sp. (strain CcI3) [Complete proteome] [HAMAP] EMBL ABD13231.1

Taxonomic identifier

106370 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family. HAMAP MF_00191

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00191
Ligand	3Fe-4S HAMAP MF_00191 Iron Iron-sulfur Metal-binding NADP HAMAP MF_00191
Molecular function	Oxidoreductase HAMAP MF_00191 EMBL ABD13231.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q2J661 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 8527C50B72852491	FASTA	342	36,955
	10 20 30 40 50 60 MSPSGDASPS RLPGRPPPRR LPPMGRVLLA KPRGYCAGVD RAVVTVEKAL ELYGSPVYVR 70 80 90 100 110 120 KQIVHNLHVV KTLEAKGAIF VDETDEVPHG ATVVFSAHGV APTVHEEAAV RELRTIDATC 130 140 150 160 170 180 PLVTKVHSEA RRFAREDYDI LLIGHEGHEE VVGTTGQAPD RIHLVDGPED AAGVKVRDPE 190 200 210 220 230 240 RVAFLSQTTL SVDETMTTVD ALRERFPHLQ GPPSDDICYA TQNRQVAVKE IAGAVDLVIV 250 260 270 280 290 300 VGSRNSSNSV RLVEVALDAG APAAYLVDDS TEVDLSWFDG VETVGVTSGA SVPEELVTGV 310 320 330 340 MAWLAERGFT DVEEVTSADE HLLFALPPEL RREMRTRERA AG

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000249 Genomic DNA. Translation: ABD13231.1.
RefSeq	YP_482960.1. NC_007777.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2J661.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3906649.
GenomeReviews	Gene locus Francci3_3881 in contig CP000249_GR.
KEGG	fra:Francci3_3881.
PATRIC	21931238. VBIFraSp10456_4222.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	AMAPECD.
PhylomeDB	Q2J661.
ProtClustDB	PRK01045.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q2J661_FRASC

Accession

Primary (citable) accession number: Q2J661

Entry history

Integrated into UniProtKB/TrEMBL:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	December 14, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)