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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Frankia sp. (strain CcI3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821SubstrateUniRule annotation
Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
Metal bindingi230 – 2301ManganeseUniRule annotation
Binding sitei230 – 2301SubstrateUniRule annotation
Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
Binding sitei272 – 2721SubstrateUniRule annotation
Active sitei274 – 2741UniRule annotation
Metal bindingi297 – 2971ManganeseUniRule annotation
Binding sitei389 – 3891GTPUniRule annotation
Binding sitei420 – 4201GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi273 – 2786GTPUniRule annotation
Nucleotide bindingi515 – 5184GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciFSP106370:GI1F-3948-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Francci3_3897
OrganismiFrankia sp. (strain CcI3)
Taxonomic identifieri106370 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia
ProteomesiUP000001937 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 609609Phosphoenolpyruvate carboxykinase [GTP]PRO_1000060290Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi106370.Francci3_3897.

Structurei

3D structure databases

ProteinModelPortaliQ2J645.
SMRiQ2J645. Positions 13-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 3893Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2J645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTAAQVSGL DVAPTRHARL VAWVREIAEL TQPDRVEWCD GSEAEFDRLT
60 70 80 90 100
SLLIEQGTLV RLNDEKRPNS FYAASDPGDV ARVEDRTYIC SEQAEDAGPT
110 120 130 140 150
NNWLDPAEMR ATLRGLFAGC MRGRTMYVVP FCMGPLGSRI SALGVEITDS
160 170 180 190 200
PYVVISMRTM TRMGTPALEQ LGTDGFFVPA VHSLGAPLEP GQADVPWPCN
210 220 230 240 250
TTKYITHFPE TREIWSYGSG YGGNALLGKK CYALRIASVM ARDEGWLAEH
260 270 280 290 300
MLILKLTSPA GKVHYIAAAF PSACGKTNLA MLIPTLPGWQ AETVGDDIAW
310 320 330 340 350
MRFGEDGRLY AVNPEAGFFG VAPGTGEQTN PNAVRTLWGN AIYTNVARTD
360 370 380 390 400
NGDVWWEGLT KQPPAHLIDW KGRDWTPESS EPAAHPNARF TVPAGQCPTI
410 420 430 440 450
APEWEDPRGV PISAILFGGR RATAVPLVTE APDWRRGVFF GSIVASETTA
460 470 480 490 500
AQAGAIGKLR RDPFAMLPFC GYNMADYFAH WLEVGQKADQ TKLPRVYYVN
510 520 530 540 550
WFRKSPEGRF LWPGFGDNSR VLAWIVGRLE GTAAGVETPL GVLPTKDALP
560 570 580 590 600
VDGIDIAEED LETLLTVDVE VWKQEAQLIP EHYQTFGERL PAALWTEHEA

LVERLNSAD
Length:609
Mass (Da):66,912
Last modified:March 6, 2006 - v1
Checksum:i3790D69E45471916
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD13247.1.
RefSeqiYP_482976.1. NC_007777.1.

Genome annotation databases

EnsemblBacteriaiABD13247; ABD13247; Francci3_3897.
KEGGifra:Francci3_3897.
PATRICi21931270. VBIFraSp10456_4238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD13247.1.
RefSeqiYP_482976.1. NC_007777.1.

3D structure databases

ProteinModelPortaliQ2J645.
SMRiQ2J645. Positions 13-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi106370.Francci3_3897.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD13247; ABD13247; Francci3_3897.
KEGGifra:Francci3_3897.
PATRICi21931270. VBIFraSp10456_4238.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciFSP106370:GI1F-3948-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CcI3.

Entry informationi

Entry nameiPCKG_FRASC
AccessioniPrimary (citable) accession number: Q2J645
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 4, 2008
Last sequence update: March 6, 2006
Last modified: March 31, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.