Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q2J5A7 (BIOB2_FRASC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase 2

EC=2.8.1.6
Gene names
Name:bioB2
Ordered Locus Names:Francci3_4187
OrganismFrankia sp. (strain CcI3) [Complete proteome] [HAMAP]
Taxonomic identifier106370 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence ABD13535.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360Biotin synthase 2 HAMAP-Rule MF_01694
PRO_0000381389

Sites

Metal binding681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding721Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1121Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1451Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2051Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2751Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2J5A7 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: C7F5311FA2E4F53D

FASTA36038,258
        10         20         30         40         50         60 
MDLSATLNSL VSKGVSGQAP TRDEALAVLR SDDDDLLDVV AAAYRLRRRY FGRRVKLNFL 

        70         80         90        100        110        120 
VNLKSGLCPE DCSYCSQRLG SNTGILKYTW LKPEEAAATA GAGISGGARR VCLVASGRGP 

       130        140        150        160        170        180 
TDRDVDRVAD TIGAIKTAHP DVEVCACLGL LSDGQAAQLR AAGADAYNHN LNTAGEKYAD 

       190        200        210        220        230        240 
ICTTHTYNDR VDTVQEARHA GLSPCSGIIA GMGESDEDLV DVAFALRELA PDSIPVNFLM 

       250        260        270        280        290        300 
PFEGTPLGAE WNLNPRQCLR ILAMVRFVNP TAEVRLSGGR EIHLGSMQPL ALSVVNSIFL 

       310        320        330        340        350        360 
GDYLTSEGQE GHQDLKMIAE AGFTVEGLNT DAEAALAMGA GLERVALRQR GAGTDLPPNA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000249 Genomic DNA. Translation: ABD13535.1. Different initiation.
RefSeqYP_483264.1. NC_007777.1.

3D structure databases

ProteinModelPortalQ2J5A7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING106370.Francci3_4187.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD13535; ABD13535; Francci3_4187.
GeneID3907152.
KEGGfra:Francci3_4187.
PATRIC21931888. VBIFraSp10456_4543.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OrthoDBEOG622PMP.
ProtClustDBPRK06256.

Enzyme and pathway databases

BioCycFSP106370:GI1F-4245-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB2_FRASC
AccessionPrimary (citable) accession number: Q2J5A7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways