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Q2J5A7

- BIOB2_FRASC

UniProt

Q2J5A7 - BIOB2_FRASC

Protein

Biotin synthase 2

Gene

bioB2

Organism
Frankia sp. (strain CcI3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi112 – 1121Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi145 – 1451Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi205 – 2051Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi275 – 2751Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciFSP106370:GI1F-4245-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthase 2UniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioB2UniRule annotation
    Ordered Locus Names:Francci3_4187
    OrganismiFrankia sp. (strain CcI3)
    Taxonomic identifieri106370 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia
    ProteomesiUP000001937: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360Biotin synthase 2PRO_0000381389Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi106370.Francci3_4187.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2J5A7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2J5A7-1 [UniParc]FASTAAdd to Basket

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    MDLSATLNSL VSKGVSGQAP TRDEALAVLR SDDDDLLDVV AAAYRLRRRY    50
    FGRRVKLNFL VNLKSGLCPE DCSYCSQRLG SNTGILKYTW LKPEEAAATA 100
    GAGISGGARR VCLVASGRGP TDRDVDRVAD TIGAIKTAHP DVEVCACLGL 150
    LSDGQAAQLR AAGADAYNHN LNTAGEKYAD ICTTHTYNDR VDTVQEARHA 200
    GLSPCSGIIA GMGESDEDLV DVAFALRELA PDSIPVNFLM PFEGTPLGAE 250
    WNLNPRQCLR ILAMVRFVNP TAEVRLSGGR EIHLGSMQPL ALSVVNSIFL 300
    GDYLTSEGQE GHQDLKMIAE AGFTVEGLNT DAEAALAMGA GLERVALRQR 350
    GAGTDLPPNA 360
    Length:360
    Mass (Da):38,258
    Last modified:July 28, 2009 - v2
    Checksum:iC7F5311FA2E4F53D
    GO

    Sequence cautioni

    The sequence ABD13535.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000249 Genomic DNA. Translation: ABD13535.1. Different initiation.
    RefSeqiYP_483264.1. NC_007777.1.

    Genome annotation databases

    EnsemblBacteriaiABD13535; ABD13535; Francci3_4187.
    GeneIDi3907152.
    KEGGifra:Francci3_4187.
    PATRICi21931888. VBIFraSp10456_4543.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000249 Genomic DNA. Translation: ABD13535.1 . Different initiation.
    RefSeqi YP_483264.1. NC_007777.1.

    3D structure databases

    ProteinModelPortali Q2J5A7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 106370.Francci3_4187.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD13535 ; ABD13535 ; Francci3_4187 .
    GeneIDi 3907152.
    KEGGi fra:Francci3_4187.
    PATRICi 21931888. VBIFraSp10456_4543.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci FSP106370:GI1F-4245-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CcI3.

    Entry informationi

    Entry nameiBIOB2_FRASC
    AccessioniPrimary (citable) accession number: Q2J5A7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3