Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q2J4Z1 (Q2J4Z1_FRASC) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesSubmitted name:
DNA topoisomerase I EMBL ABD13651.1
EC=5.99.1.2 EMBL ABD13651.1
Gene names
Ordered Locus Names:Francci3_4305
OrganismFrankia sp. (strain CcI3) [Complete proteome] [HAMAP] EMBL ABD13651.1
Taxonomic identifier106370 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length1049 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733

Sequence similarities

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q2J4Z1 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: FE8C54E3D379716A

FASTA1,049111,951
        10         20         30         40         50         60 
MPARTKTTTR ATARSSAPVA EPTEAGLPPE TGSSEASADV PAGATSRTAN GRTEAGHSAN 

        70         80         90        100        110        120 
GSGGATGAHG SRATGSGPGN RLVIVESPAK AKTIAGYLGP GWQVESSIGH IRDLPRSAAD 

       130        140        150        160        170        180 
VPAAHKGKPW ARLGVDVDND FEPLYIVTPD KKPQVSKLKA LVKDASELYL ATDEDREGEA 

       190        200        210        220        230        240 
IAWHLLQTLK PTVPVKRMVF HEITPQAIQR AVDNPRDIDK NLVNAQETRR ILDRLYGYEV 

       250        260        270        280        290        300 
SPVLWKKVMP RLSAGRVQSV ATRILVERER ARMRFHSAEY WNIEGLFGAT VARQAWSGAD 

       310        320        330        340        350        360 
GTPGSGPDGP GAGSIRGDGV EKTPLPATLI ALNGNRIATG RDFSPTGQLV SSGVTRLDEA 

       370        380        390        400        410        420 
TARSLAERLA DAAFTVRSVE TKPYRRSPYP PFMTSTLQQE AGRKLRFSSQ RTMQVAQRLY 

       430        440        450        460        470        480 
ENGYITYMRT DSTNLSKTAL TAARAQAASL YGPEYVPARP RTYAKKVKNA QEAHEAIRPA 

       490        500        510        520        530        540 
GDHFRTPGEV RGELDVDSYR LYELIWQRTV ASQMADARGT SATIRLGATS SAGEDAEFSA 

       550        560        570        580        590        600 
SGKVITFPGF LRAYVEGADD PDAELEDRER RLPDVRQGDP LTTRSLTPRG HTTSPPARFT 

       610        620        630        640        650        660 
EASLVKTLEE LGIGRPSTYA SIIGTIQDRG YVWKKGSALV PSFVAFAVVG LLEDHFTRLV 

       670        680        690        700        710        720 
DYRFTATMED DLDDIAAGTA ASTDWLTRFY FGTGDGTDPA ADGLKHLVNE RLGEIDAREV 

       730        740        750        760        770        780 
NSIPLGETDD GTLLVVRVGR YGPYVQHGER RASVPDDLAP DELTVDKALG LLAAPSGDRM 

       790        800        810        820        830        840 
LGIDPASGAT ITAKAGRFGP YVTTDTDPPR TSSLLRGMSL ETLTLDDAVR LLTLPRILGA 

       850        860        870        880        890        900 
GDDGEEVTAQ NGRYGPYVKK GAESRSLESE DQLFTVTLDE ALALLSQPKA RGRRQAAQSP 

       910        920        930        940        950        960 
PLRELGTDPA SGKPMVVREG RFGPYVTDGE TNASLRKGDT VETITDERAA ELLADRRARG 

       970        980        990       1000       1010       1020 
PATAKRPARG TAKAGTAKTG PKTTKAKPDT AKSGTAKSGT AKTGTAKTGT AKTGTARSKT 

      1030       1040 
ARTVTDDGGG SDGSDDSGSS SSSGTRRTD

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000249 Genomic DNA. Translation: ABD13651.1.
RefSeqYP_483380.1. NC_007777.1.

3D structure databases

ProteinModelPortalQ2J4Z1.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2J4Z1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3907273.
GenomeReviewsGene locus Francci3_4305 in contig CP000249_GR.
KEGGfra:Francci3_4305.
PATRIC21932159. VBIFraSp10456_4677.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0550.
HOGENOMHBG512935.
OMAKKVMPRL.
PhylomeDBQ2J4Z1.
ProtClustDBCLSK949032.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 3 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ2J4Z1_FRASC
AccessionPrimary (citable) accession number: Q2J4Z1
Entry history
Integrated into UniProtKB/TrEMBL: March 7, 2006
Last sequence update: March 7, 2006
Last modified: January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info