Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitrogenase iron protein

Gene

nifH

Organism
Frankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.UniRule annotation

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi96Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi130Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrogen fixation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron proteinUniRule annotation (EC:1.18.6.1UniRule annotation)
Alternative name(s):
Nitrogenase Fe proteinUniRule annotation
Nitrogenase component IIUniRule annotation
Nitrogenase reductaseUniRule annotation
Gene namesi
Name:nifHUniRule annotation
Ordered Locus Names:Francci3_4488
OrganismiFrankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3)
Taxonomic identifieri106370 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000001937 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002118701 – 287Nitrogenase iron proteinAdd BLAST287

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei99ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseUniRule annotation1

Post-translational modificationi

The reversible ADP-ribosylation of Arg-99 inactivates the nitrogenase reductase and regulates nitrogenase activity.UniRule annotation

Keywords - PTMi

ADP-ribosylation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi106370.Francci3_4488.

Structurei

3D structure databases

ProteinModelPortaliQ2J4F8.
SMRiQ2J4F8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228826.
KOiK02588.
OMAiNKMFVIP.
OrthoDBiPOG091H0230.

Family and domain databases

CDDicd02040. NifH. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiView protein in InterPro
IPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
PANTHERiPTHR42864. PTHR42864. 1 hit.
PfamiView protein in Pfam
PF00142. Fer4_NifH. 1 hit.
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiView protein in PROSITE
PS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2J4F8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQIAFYGKG GIGKSTTQQN TMAAMAEMGK KVMIVGCDPK ADSTRLILHS
60 70 80 90 100
KAQTSVIQLA AEKGSVEDLE LDEVLVEGQW GIKCVESGGP EPGVGCAGRG
110 120 130 140 150
VITSISYLEE AGAYEDLDFV TYDVLGDVVC GGFAMPIRQG KAQEIYIVTS
160 170 180 190 200
GEMMAMYAAN NIARGILKYA HSGGVRLGGL ICNSRNTDRE DELIIELARR
210 220 230 240 250
LNTQMIHFIP RNNVVQHAEL RRMTVIEYDP KNEQADQYRQ LAKKIVDNDM
260 270 280
KTIPTPITMD ELEELLIEFG IMEQEDESII GKAAAVA
Length:287
Mass (Da):31,423
Last modified:March 7, 2006 - v1
Checksum:iE2798FFCDCDB77D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD13834.1.
RefSeqiWP_011438842.1. NC_007777.1.

Genome annotation databases

EnsemblBacteriaiABD13834; ABD13834; Francci3_4488.
KEGGifra:Francci3_4488.
PATRICi21932589. VBIFraSp10456_4887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000249 Genomic DNA. Translation: ABD13834.1.
RefSeqiWP_011438842.1. NC_007777.1.

3D structure databases

ProteinModelPortaliQ2J4F8.
SMRiQ2J4F8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi106370.Francci3_4488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD13834; ABD13834; Francci3_4488.
KEGGifra:Francci3_4488.
PATRICi21932589. VBIFraSp10456_4887.

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228826.
KOiK02588.
OMAiNKMFVIP.
OrthoDBiPOG091H0230.

Family and domain databases

CDDicd02040. NifH. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiView protein in InterPro
IPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
PANTHERiPTHR42864. PTHR42864. 1 hit.
PfamiView protein in Pfam
PF00142. Fer4_NifH. 1 hit.
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiView protein in PROSITE
PS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNIFH_FRACC
AccessioniPrimary (citable) accession number: Q2J4F8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 7, 2006
Last modified: March 15, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.