Reviewed, UniProtKB/Swiss-Prot Q2J2X6 (ACEK_RHOP2)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	RPB_0473

Organism

Rhodopseudomonas palustris (strain HaA2) [Complete proteome] [HAMAP]

Taxonomic identifier

316058 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 605	605	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288300
Regions
	Nucleotide binding	353 – 359	7	ATP By similarity
Sites
	Active site	413	1	By similarity
	Binding site	374	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2J2X6-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 80A85EEA62C1DC74	FASTA	605	67,293
	10 20 30 40 50 60 MTATRHPFAS DIDAATRIAE PDFDLLDALV RTDDPDDQAH TLARVVLAAF DNYYAVSRRI 70 80 90 100 110 120 PALAQAAFEA RDWAATVRLS KIRLGLYTAC IDQLVPLLKA GLPELANDEQ LWARAEAELL 130 140 150 160 170 180 AAIAERYEAD FAFAFWQSLR RKLVSDEWRP VSYDTGPAAR PKATSAAILK TIATTLPIRP 190 200 210 220 230 240 AVIRDILDGA GLRGPWRDRD GDAALAAAAI EAALEPLGPR AGETAKIEIA ESGFFRNRGA 250 260 270 280 290 300 CLVGRVRLRD RGDMPMRNLP LLIALLNEDD GLVVDAVLCD ADELQYAFSS TLANYHATNP 310 320 330 340 350 360 HYHELARLLH ELMPKRPLGT QYSCIGFHHL GKVAVMTEIL AEHRRSKEKL DTAPGFKGTV 370 380 390 400 410 420 AIAFTMPSSA YVLKIIRDHP TDDYKFDYFD GLDAVLRKYN LVHEIDRAGS MLDNIIYSNV 430 440 450 460 470 480 KLERTMFAPD LLDELLESGI DTVTLERGAL VFRHLIVQIK LTPLPLYLTT ASAADARAAV 490 500 510 520 530 540 INLGDCIKNN AAADIFNKDL DGRNYGVSRI RKVYLFDYDA VEPLTDVRVR GDDAPPGPEF 550 560 570 580 590 600 EDGVVFRPAD MLGGLRIDDP ALRRAFRDAH PELMQPEYWQ GMQRALRAGK VPRVMNYPAA RRLRR

References

[1]

"Complete sequence of Rhodopseudomonas palustris HaA2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Pelletier D.A. , Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000250 Genomic DNA. Translation: ABD05184.1.
RefSeq	YP_484095.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2J2X6.
Genome annotation databases
GeneID	3909818.
GenomeReviews	Gene locus RPB_0473 in contig CP000250_GR.
KEGG	rpb:RPB_0473.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2J2X6.
OMA	FYRDFLH.
Enzyme and pathway databases
BioCyc	RPAL316058:RPB_0473-MON.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_RHOP2

Accession

Primary (citable) accession number: Q2J2X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)