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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Rhodopseudomonas palustris (strain HaA2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei67ShikimateUniRule annotation1
Active sitei71Proton acceptorUniRule annotation1
Binding sitei82NADPUniRule annotation1
Binding sitei91ShikimateUniRule annotation1
Binding sitei106ShikimateUniRule annotation1
Binding sitei220NADP; via carbonyl oxygenUniRule annotation1
Binding sitei222ShikimateUniRule annotation1
Binding sitei243NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi131 – 135NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciRPAL316058:G1G5V-1312-MONOMER
UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:RPB_1302
OrganismiRhodopseudomonas palustris (strain HaA2)
Taxonomic identifieri316058 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000008809 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000213211 – 280Shikimate dehydrogenase (NADP(+))Add BLAST280

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi316058.RPB_1302

Structurei

3D structure databases

ProteinModelPortaliQ2J0J8
SMRiQ2J0J8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 22Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237875
KOiK00014
OMAiIPHKERA
OrthoDBiPOG091H02DY

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

Q2J0J8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPGDGHTAA CLIGWPAAHS RSPLIHHYWL RTLGIPGGYS IESVPPEGFA
60 70 80 90 100
EFVLNLRAHG YSGANVTIPH KERALQLTQP DDRARAVGAA NTLYYDGDVL
110 120 130 140 150
RSTNTDVEGF IANLDASAPG WDRTPHAVVL GAGGSARAVL FGLLERGIER
160 170 180 190 200
IALANRSLER AQALADLFGE RVVPIAWTDA PAALPGAGLL VNTTSLGMKG
210 220 230 240 250
QPSLDLDLEP LPSDATVADL VYVPLETELL AEARGRGLRT ADGLGMLLHQ
260 270 280
AVRGFELWFG ARPHVTAELR ALVEADLAAK
Length:280
Mass (Da):29,776
Last modified:March 7, 2006 - v1
Checksum:iC34B059DDD174A25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000250 Genomic DNA Translation: ABD06012.1
RefSeqiWP_011440200.1, NC_007778.1

Genome annotation databases

EnsemblBacteriaiABD06012; ABD06012; RPB_1302
KEGGirpb:RPB_1302

Similar proteinsi

Entry informationi

Entry nameiAROE_RHOP2
AccessioniPrimary (citable) accession number: Q2J0J8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: April 25, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health