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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Rhodopseudomonas palustris (strain HaA2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671ShikimateUniRule annotation
Active sitei71 – 711Proton acceptorUniRule annotation
Binding sitei82 – 821NADPUniRule annotation
Binding sitei91 – 911ShikimateUniRule annotation
Binding sitei106 – 1061ShikimateUniRule annotation
Binding sitei220 – 2201NADP; via carbonyl oxygenUniRule annotation
Binding sitei222 – 2221ShikimateUniRule annotation
Binding sitei243 – 2431NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi131 – 1355NADPUniRule annotation

GO - Molecular functioni

  1. NADP binding Source: InterPro
  2. shikimate 3-dehydrogenase (NADP+) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. aromatic amino acid family biosynthetic process Source: UniProtKB-HAMAP
  2. chorismate biosynthetic process Source: UniProtKB-UniPathway
  3. shikimate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciRPAL316058:GHF1-1320-MONOMER.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:RPB_1302
OrganismiRhodopseudomonas palustris (strain HaA2)
Taxonomic identifieri316058 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000008809 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Shikimate dehydrogenase (NADP(+))PRO_1000021321Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi316058.RPB_1302.

Structurei

3D structure databases

ProteinModelPortaliQ2J0J8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 223Shikimate bindingUniRule annotation

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0169.
HOGENOMiHOG000237875.
KOiK00014.
OMAiTTVDGAW.
OrthoDBiEOG64R67G.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_quinate_DH.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2J0J8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPGDGHTAA CLIGWPAAHS RSPLIHHYWL RTLGIPGGYS IESVPPEGFA
60 70 80 90 100
EFVLNLRAHG YSGANVTIPH KERALQLTQP DDRARAVGAA NTLYYDGDVL
110 120 130 140 150
RSTNTDVEGF IANLDASAPG WDRTPHAVVL GAGGSARAVL FGLLERGIER
160 170 180 190 200
IALANRSLER AQALADLFGE RVVPIAWTDA PAALPGAGLL VNTTSLGMKG
210 220 230 240 250
QPSLDLDLEP LPSDATVADL VYVPLETELL AEARGRGLRT ADGLGMLLHQ
260 270 280
AVRGFELWFG ARPHVTAELR ALVEADLAAK
Length:280
Mass (Da):29,776
Last modified:March 7, 2006 - v1
Checksum:iC34B059DDD174A25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000250 Genomic DNA. Translation: ABD06012.1.
RefSeqiYP_484923.1. NC_007778.1.

Genome annotation databases

EnsemblBacteriaiABD06012; ABD06012; RPB_1302.
KEGGirpb:RPB_1302.
PATRICi23297165. VBIRhoPal125544_1378.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000250 Genomic DNA. Translation: ABD06012.1.
RefSeqiYP_484923.1. NC_007778.1.

3D structure databases

ProteinModelPortaliQ2J0J8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316058.RPB_1302.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD06012; ABD06012; RPB_1302.
KEGGirpb:RPB_1302.
PATRICi23297165. VBIRhoPal125544_1378.

Phylogenomic databases

eggNOGiCOG0169.
HOGENOMiHOG000237875.
KOiK00014.
OMAiTTVDGAW.
OrthoDBiEOG64R67G.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BioCyciRPAL316058:GHF1-1320-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_quinate_DH.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HaA2.

Entry informationi

Entry nameiAROE_RHOP2
AccessioniPrimary (citable) accession number: Q2J0J8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: April 1, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.