Reviewed, UniProtKB/Swiss-Prot Q2J0A5 (MASZ_RHOP2)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	RPB_1395

Organism

Rhodopseudomonas palustris (strain HaA2) [Complete proteome] [HAMAP]

Taxonomic identifier

316058 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Malate synthase G HAMAP MF_00641		PRO_1000056923
Sites
	Active site	337	1	Proton acceptor By similarity
	Active site	628	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2J0A5-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 2D5D28519CD7EE0A	FASTA	724	78,443
	10 20 30 40 50 60 MNRIDAHGLK IAPVLFDFIA KEAAPKTGVA PDAFWAGLAA IVRDLTPKTV ALLQHRDGLQ 70 80 90 100 110 120 AKIDAWHLAN KGKKQDMAAY TAFLKEIGYL LPEPPTVAVE TANVDDEIGK LCGPQLVVPL 130 140 150 160 170 180 SNARYALNAA NARWGSLYDA FYGTDAIPQE ASQAKGYDKA RGDKVIAKAK AFLDQAAPLV 190 200 210 220 230 240 AGSHSDVTAY SVIAGQLSAK LKSGNATGLK TPRQFAGYLG DAASPSAVLL VNNGLHIEIK 250 260 270 280 290 300 IDRANTIGKD DAAGVADLVI ESAVSTILDM EDSVAAVDAE DKVLIYRNTL GLMDGTLSAD 310 320 330 340 350 360 FDKGGKTVTR ALNGDRTYTG PDGKDVTLHG RSLLLMRNVG HHMWTDAVLD SNGDEIPEGF 370 380 390 400 410 420 LDAAVSGLLA IHDLKALGKT RNSRTGSVYI VKPKMHGPDE VALTCELFGR VEQMLGLKEN 430 440 450 460 470 480 TLKVGIMDEE RRTTVNLKAC IQNASKRIVF INTGFLDRTG DEIHTSMEAG PMIRKNEMKA 490 500 510 520 530 540 QPWIKAYEDW NVDTGLIDGL PGHAQIGKGM WAAPDKMADM LTQKIGHPQA GATTAWVPSP 550 560 570 580 590 600 TAATLHALHY HQVDVLARQQ ELKTGGPRAK LEDILTIPVS QSNWAPDDVR QEIDNNCQGI 610 620 630 640 650 660 LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHHGVVTK EQVLESLKRM 670 680 690 700 710 720 AAVVDKQNAG DPLYRPMAPD FDGVAFEAAC DLIFKGREQP NGYTEFILHI RRREAKAAHL QDLT

References

[1]

"Complete sequence of Rhodopseudomonas palustris HaA2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Pelletier D.A. , Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000250 Genomic DNA. Translation: ABD06105.1.
RefSeq	YP_485016.1.
3D structure databases
SMR	Q2J0A5. Positions 2-717.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2J0A5.
Genome annotation databases
GeneID	3908345.
GenomeReviews	Gene locus RPB_1395 in contig CP000250_GR.
KEGG	rpb:RPB_1395.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HBG350005.
OMA	PKMHGPD.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_RHOP2

Accession

Primary (citable) accession number: Q2J0A5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)