Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q2IZ35 (Q2IZ35_RHOP2) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
tRNA(Ile)-lysidine synthase HAMAP-Rule MF_01161
EC=6.3.4.19 HAMAP-Rule MF_01161
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase HAMAP-Rule MF_01161
tRNA(Ile)-lysidine synthetase HAMAP-Rule MF_01161
Gene names
Name:tilS HAMAP-Rule MF_01161
Ordered Locus Names:RPB_1817
OrganismRhodopseudomonas palustris (strain HaA2) [Complete proteome] [HAMAP] EMBL ABD06525.1
Taxonomic identifier316058 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas ›

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity. HAMAP-Rule MF_01161 SAAS SAAS011063

Catalytic activity

(tRNA(Ile2))-cytidine34 + L-lysine + ATP = (tRNA(Ile2))-lysidine34 + AMP + diphosphate + H2O. HAMAP-Rule MF_01161 SAAS SAAS011063

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01161.

Domain

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding By similarity. HAMAP-Rule MF_01161

Sequence similarities

Belongs to the tRNA(Ile)-lysidine synthase family. HAMAP-Rule MF_01161

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding32 – 376ATP By similarity HAMAP-Rule MF_01161

Sequences

Sequence LengthMass (Da)Tools
Q2IZ35 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: A8BFAFAD4E9D9F5F

FASTA34636,970
        10         20         30         40         50         60 
MSGTDEAPIS AREASSLFAD WKAVPAIVLA VSGGPDSLAL MWLAARWRKA RKRGPALFVV 

        70         80         90        100        110        120 
TIDHGLRPEA AREARSVKQF ARTLGLPHRT LRWTGDKPAT GLPAAARAAR YRLLAKAAKA 

       130        140        150        160        170        180 
VGAAHIMTAH TRDDQAETVL MRLSRGSGIA GLAAMPRETA RDGAVLVRPL LDVSKQRLVA 

       190        200        210        220        230        240 
TLDRAKIGFA VDPSNADPRF TRPRLRELMP LLAAEGCDSR NLARLAARAA RAEAALELLV 

       250        260        270        280        290        300 
DGAERFLAVR SAGHPPQSFD ASVFYALNAE IRIRLLLRAI GRVGREGRPE LGKVEALAAA 

       310        320        330        340 
LQSAAAKPAA KPGQIKLKQT LAGAIITLTP VCLKFAAAPM RRRKTG

« Hide

References

[1]"Complete sequence of Rhodopseudomonas palustris HaA2."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Pelletier D.A. expand/collapse author list , Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HaA2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000250 Genomic DNA. Translation: ABD06525.1.
RefSeqYP_485436.1. NC_007778.1.

3D structure databases

ProteinModelPortalQ2IZ35.
ModBaseSearch...

Protein-protein interaction databases

STRING316058.RPB_1817.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD06525; ABD06525; RPB_1817.
GeneID3908976.
KEGGrpb:RPB_1817.
PATRIC23298231. VBIRhoPal125544_1909.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0037.
HOGENOMHOG000236508.
KOK04075.
OMAMWLAARW.
ProtClustDBCLSK911678.

Enzyme and pathway databases

BioCycRPAL316058:GHF1-1877-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_01161. tRNA_Ile__lys_synt.
InterProIPR012094. Lysidine-tRNA-synth.
IPR012795. Lysidine-tRNA-synth_N.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view]
PANTHERPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamPF01171. ATP_bind_3. 1 hit.
[Graphical view]
TIGRFAMsTIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ2IZ35_RHOP2
AccessionPrimary (citable) accession number: Q2IZ35
Entry history
Integrated into UniProtKB/TrEMBL: March 7, 2006
Last sequence update: March 7, 2006
Last modified: May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info