Q2IXS0 (RPOB_RHOP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	RPB_2285

Organism

Rhodopseudomonas palustris (strain HaA2) [Complete proteome] [HAMAP]

Taxonomic identifier

316058 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	1390 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1390	1390	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000300390

Sequences

	Sequence		Length	Mass (Da)
	Q2IXS0 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: D55ED38BDB01B4C8	FASTA	1,390	155,046
	10 20 30 40 50 60 MAQQTFTGRK RVRKFFGHIR EVAEMPNLIE VQKASYDQFL MVAEPPGGRD DEGLQAVFRS 70 80 90 100 110 120 VFPISDFSNA SMLEFVRYEF EPPKYDVDEC RQRGMTYAAP LKVTLRLIVF DIDEETGARS 130 140 150 160 170 180 VKDIKEQDVY MGDIPLMTMN GTFVVNGTER VIVSQMHRSP GVFFDHDKGK THSSGKLLFA 190 200 210 220 230 240 ARVIPYRGSW LDIEFDAKDI VFARIDRRRK IPVTSLMFAL GLDGEEILST FYNKILYKRT 250 260 270 280 290 300 KEGWRVPFDA SRFRGYSTVN DLIDADTGKV VLEAGKKLTV RAARQLQEKG LKALRLSDSE 310 320 330 340 350 360 LVGNYLAEDL VNPKTGEIFA EAGDELTGKP IKGDDKEIEL FVGATPMKAI LEQGYKELPL 370 380 390 400 410 420 LDIDHVNVGP YIRNTLSADK NMTREDALFD IYRVMRPGEP PTLDSAQNMF QSLFFDAERY 430 440 450 460 470 480 DLSAVGRVKM NMRLDLDAPD THRTLRKEDI LAVIKTLVGL RDGKGEIDDI DHLGNRRVRS 490 500 510 520 530 540 VGELMENQYR IGLLRMERAI KERMSSVDID TVMPQDLINA KPAAAAVREF FGSSQLSQFM 550 560 570 580 590 600 DQTNPLSEIT HKRRLSALGP GGLTRERAGF EVRDVHPTHY GRICPIETPE GPNIGLINSL 610 620 630 640 650 660 ATFARVNKYG FVETPYRKVK EGRVTDEVVY LSAMEEGRYA VAQANISLDA KGRFTDDLIV 670 680 690 700 710 720 CRAGGTRDVV LIPADQVDYM DVSPKQLVSV AAALIPFLEN DDANRALMGS NMQRQAVPLV 730 740 750 760 770 780 RAEAPFVGTG MEGVVARDSG AAIAARRTGV IDQIDATRIV IRATEDLDPT KSGVDIYRLM 790 800 810 820 830 840 KYQRSNQSTC INQRPLVKVG DKVAKGDIIA DGPSTDLGEL ALGRNVLVAF MPWNGYNFED 850 860 870 880 890 900 SILLSERIVK EDVFTSIHIE EFEVMARDTK LGPEEITRDI PNVSEEALKN LDEAGIVYIG 910 920 930 940 950 960 AEVRAGDILV GKITPKGESP MTPEEKLLRA IFGEKASDVR DTSLRVPPGV QGTIVEVRVF 970 980 990 1000 1010 1020 NRHGVDKDER ALAIEREEIE RLAKDRDDEQ AILDRNVYGR LADLLDNRQG VAGPKGFKKD 1030 1040 1050 1060 1070 1080 TKITRAVLEE YPKSQWWLFA SPNDKLMAEI EAMRKQYDES KKGLEQRFLD KVEKLQRGDE 1090 1100 1110 1120 1130 1140 LPPGVMKMVK VFVAVKRKIQ PGDKMAGRHG NKGVVSKIVP IEDMPFLEDG THADIVLNPL 1150 1160 1170 1180 1190 1200 GVPSRMNVGQ ILETHLGWAC AGMGKKIGQT IDAYYQRQDL KPLRETLKKI YGDDETIKSL 1210 1220 1230 1240 1250 1260 DDGELIELGR NLSHGVPIAT PVFDGAKEAD IEEMLKLAGF DASGQSTVYD GRTGDEFDRK 1270 1280 1290 1300 1310 1320 VTVGYIYMLK LHHLVDDKIH ARSIGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAA 1330 1340 1350 1360 1370 1380 YTLQEMLTVK SDDVAGRTKV YEAIVRGDDT FEAGIPESFN VLVKEMRSLG LNVDLHNSKL 1390 GVPPPAEAAE

References

[1]

"Complete sequence of Rhodopseudomonas palustris HaA2." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Pelletier D.A. , Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HaA2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000250 Genomic DNA. Translation: ABD06990.1.
RefSeq	YP_485901.1. NC_007778.1.
3D structure databases
HSSP	HSSP built from PDB template 2GHO based on UniProtKB Q9KWU7.
ProteinModelPortal	Q2IXS0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IXS0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3909666.
GenomeReviews	Gene locus RPB_2285 in contig CP000250_GR.
KEGG	rpb:RPB_2285.
PATRIC	23299223. VBIRhoPal125544_2399.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0085.
HOGENOM	HBG285547.
OMA	QRDLTYA.
PhylomeDB	Q2IXS0.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	RPAL316058:RPB_2285-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_RHOP2

Accession

Primary (citable) accession number: Q2IXS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program