Q2IXD6 (Q2IXD6_RHOP2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ABD07124.1
EC=5.99.1.2 EMBL ABD07124.1

Gene names

Ordered Locus Names:

RPB_2419

Organism

Rhodopseudomonas palustris (strain HaA2) [Complete proteome] [HAMAP] EMBL ABD07124.1

Taxonomic identifier

316058 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	911 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q2IXD6 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: EFFE8A8FC54F1811	FASTA	911	99,153
	10 20 30 40 50 60 MNLVIVESPA KAKTINKYLG SSYEVLASFG HVRDLPAKNG SVDPDANFQM IWEIDPKAAG 70 80 90 100 110 120 RLNDIAKALK GADKLILATD PDREGEAISW HVLEVLKQKR ALKDQKVERV VFNAITKQSV 130 140 150 160 170 180 TEAMQHPREI DGALVDAYMA RRALDYLVGF TLSPVLWRKL PGARSAGRVQ SVALRLVCDR 190 200 210 220 230 240 EMEIEKFVAR EYWSLIATLT TPRGDAFEAR LVGADGKKIQ RLDIGTGAEA EDFKQAIETA 250 260 270 280 290 300 NFNVSSVEAK PARRNPYAPF TTSTLQQEAS RKLGFAPAHT MRIAQRLYEG IDIGGETTGL 310 320 330 340 350 360 ITYMRTDGVQ IDPSAITQAR KVIAEDYGSA YVPDSPRQYQ AKAKNAQEAH EAIRPTDLSR 370 380 390 400 410 420 RPSEVNKRLD SDQARLYELI WVRTVASQME SAEMERTTVD IEAKAGSRVL ELRATGQVVK 430 440 450 460 470 480 FDGFLAAYQE GRDDDSEDED SRRLPAMSEN EALKREALAV TQHFTEPPPR FSEASLVKRM 490 500 510 520 530 540 EELGIGRPST YASILQVLKD RGYVKLEKKR LHGEDKGRVV IAFLESFFAR YVEYDFTAAL 550 560 570 580 590 600 EEKLDRISNN EISWQQVLRD FWTDFIGAVN DIKELRVAQV LDVLDEMLGP HIYAPREDGG 610 620 630 640 650 660 DPRQCPSCGT GRLNLKAGKF GAFVGCSNYP ECRHTRPLAA DGGGADADRV LGLDPDTGFE 670 680 690 700 710 720 VAVKSGRFGP YIQLGDAKDY AEGEKPKRAG IPKGTSPSDV ELDVALRLLA LPREVGKHPE 730 740 750 760 770 780 TGEPIKAGIG RFGPYVQHEK TYASLEAGDD VHNIGLNRAV TLIAEKIAKG PSKRRFGADP 790 800 810 820 830 840 GKPLGDHPSL GPVAVKAGRY GAYVTAGGVN ATIPNDKTQD TITLPEAIAL IDERAAKGGG 850 860 870 880 890 900 AKAKKKAPAK KAAAKSDAKP AKKAAAKKPK AEGAAASPAR APVKAKTSTT KPKAAAAKPK 910 SPAKKSAAKN G

References

[1]

"Complete sequence of Rhodopseudomonas palustris HaA2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Pelletier D.A. , Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HaA2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000250 Genomic DNA. Translation: ABD07124.1.
RefSeq	YP_486035.1. NC_007778.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IXD6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3909553.
GenomeReviews	Gene locus RPB_2419 in contig CP000250_GR.
KEGG	rpb:RPB_2419.
PATRIC	23299515. VBIRhoPal125544_2543.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	KKVMPRL.
PhylomeDB	Q2IXD6.
ProtClustDB	PRK07561.
Enzyme and pathway databases
BioCyc	RPAL316058:RPB_2419-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q2IXD6_RHOP2

Accession

Primary (citable) accession number: Q2IXD6

Entry history

Integrated into UniProtKB/TrEMBL:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)