Q2IX59 (SYD_RHOP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	RPB_2496

Organism

Rhodopseudomonas palustris (strain HaA2) [Complete proteome] [HAMAP]

Taxonomic identifier

316058 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000006738

Sequences

	Sequence		Length	Mass (Da)
	Q2IX59 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 12E3CB79E6202F5C	FASTA	590	66,700
	10 20 30 40 50 60 MHRYRTHTCG ALRDSDIDQT VRVSGWCHRI RDHGGLLFID LRDHYGLTQC VADPDSPAFK 70 80 90 100 110 120 DAEKLRAEWV VRIDGRVRRR PEGTDNDDLP TGKVEIFITE IEVLGPAGEL PLPVFGEQDY 130 140 150 160 170 180 PEDVRLRYRF LDLRREKLHQ NIMTRGAIVD AMRKRMKEQG FFEFQTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRIHPGK FYALPQAPQQ YKQLLMMSGF DRYFQIAPCF RDEDPRADRL PGEFYQLDVE 250 260 270 280 290 300 MSFVTQDDVF AAMEPVITGV FEDFAKGKPV TKSWPRIPYF ESLRKYGTDK PDLRNPLEMQ 310 320 330 340 350 360 DVSEHFRGSG FKVFARMLEE ERNQVWAIPG PGGGSRAFCD RMNSWAQGEG QPGLGYIMWR 370 380 390 400 410 420 EGNEGAGPLA NNIGPERTEA IRVALGLKAG DAAFFVAGDP AKFVKFAGLA RTKVGEELNL 430 440 450 460 470 480 IDKDQFALAW VVDFPMYEYN EDDKKVDFSH NPFSMPQGGM DALTSQDPLT IKAFQYDITC 490 500 510 520 530 540 NGYEIASGGI RNHRPEAMVK AFEIAGYGEQ EVIDRFGGMY RAFQYGAPPH GGMAAGVDRI 550 560 570 580 590 VMLLCGTNNL REISLFPMNQ RAEDLLMGAP SQVAPKQLRE LHIRLNLPES

References

[1]

"Complete sequence of Rhodopseudomonas palustris HaA2." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Pelletier D.A. , Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HaA2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000250 Genomic DNA. Translation: ABD07201.1.
RefSeq	YP_486112.1. NC_007778.1.
3D structure databases
ProteinModelPortal	Q2IX59.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IX59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3910285.
GenomeReviews	Gene locus RPB_2496 in contig CP000250_GR.
KEGG	rpb:RPB_2496.
PATRIC	23299681. VBIRhoPal125544_2626.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
HOGENOM	HBG396032.
OMA	YQLDVEM.
PhylomeDB	Q2IX59.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	RPAL316058:RPB_2496-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_RHOP2

Accession

Primary (citable) accession number: Q2IX59

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program