Q2IWH1 (PIMT2_RHOP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-L-isoaspartate O-methyltransferase 2
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase 2
Protein L-isoaspartyl methyltransferase 2
Protein-beta-aspartate methyltransferase 2
Short name=PIMT 2

Gene names

Name:	pcm2
Ordered Locus Names:	RPB_2737

Organism

Rhodopseudomonas palustris (strain HaA2) [Complete proteome] [HAMAP]

Taxonomic identifier

316058 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas › Rhodopseudomonas palustris

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity. HAMAP-Rule MF_00090
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP-Rule MF_00090
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Protein-L-isoaspartate O-methyltransferase 2 HAMAP-Rule MF_00090		PRO_0000351927
Sites
	Active site	64	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IWH1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 2455AE1487410539	FASTA	217	23,904
	10 20 30 40 50 60 MISSVAPPPE KMLFQLSLRR RGISDRGVLQ AMESVPRDRF VDAVHRDSAW RDTALPIACG 70 80 90 100 110 120 QTISQPFVVA YMTEQLHLQP GHRVLEIGTG SGYHAAVLSR LVRDVVSVER FKTLADRARA 130 140 150 160 170 180 RLKELNYANV EVVLGDGFAL PEGQGTFDRI LVTAAMAELP QPLLDLLDPD GILIAPIGPG 190 200 210 NGRQTLIRVQ RKDDGFLRKP LVDVRFVPAL PGIAREL

References

[1]

"Complete sequence of Rhodopseudomonas palustris HaA2." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Pelletier D.A. , Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HaA2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000250 Genomic DNA. Translation: ABD07439.1.
RefSeq	YP_486350.1. NC_007778.1.
3D structure databases
ProteinModelPortal	Q2IWH1.
ModBase	Search...
Protein-protein interaction databases
STRING	316058.RPB_2737.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD07439; ABD07439; RPB_2737.
GeneID	3910530.
KEGG	rpb:RPB_2737.
PATRIC	23300207. VBIRhoPal125544_2885.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2518.
HOGENOM	HOG000257189.
KO	K00573.
OMA	PYDAIMV.
ProtClustDB	CLSK819579.
Enzyme and pathway databases
BioCyc	RPAL316058:GHF1-2797-MONOMER.
Family and domain databases
HAMAP	MF_00090. PIMT.
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PTHR11579. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT2_RHOP2

Accession

Primary (citable) accession number: Q2IWH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	March 7, 2006
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program