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Q2IUT3

- BIOB_RHOP2

UniProt

Q2IUT3 - BIOB_RHOP2

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Protein

Biotin synthase

Gene
bioB, RPB_3331
Organism
Rhodopseudomonas palustris (strain HaA2)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi141 – 1411Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi201 – 2011Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi274 – 2741Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRPAL316058:GHF1-3375-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:RPB_3331
OrganismiRhodopseudomonas palustris (strain HaA2)
Taxonomic identifieri316058 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000008809: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353Biotin synthaseUniRule annotationPRO_0000381580Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi316058.RPB_3331.

Structurei

3D structure databases

ProteinModelPortaliQ2IUT3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2IUT3-1 [UniParc]FASTAAdd to Basket

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MNSIDLASLA AATPTLRHDW TREQAAAIYN LPFADLIFRA QTIHRQSFDA    50
NEVQCNQLLN VKTGGCAEDC GYCSQSAHHD TALPASKLMD PKKVIEGAKA 100
ARDAGATRYC MGAAWRSPKD RDMAPVIEMV KGVKALGMEA CMTLGMLTDD 150
QAQQLADAGL DYYNHNIDTS EEFYSSVVKS RSFGDRLDTL ATVQDAGIKV 200
CCGGILGLGE KPTDRVEMLR TLANLPQHPE SVPINMLIPI EGTPIAKTAK 250
PVDPFEFVRT IALARIMMPK SDVRLAAGRT AMSDEMQSLC FLAGANSIFI 300
GDTLLTTPNP GDSKDRMLFA RLGITPREGA PVEAHSHDHD HDHHDHHHGH 350
SHS 353
Length:353
Mass (Da):38,426
Last modified:March 7, 2006 - v1
Checksum:iB41B571481D9566F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000250 Genomic DNA. Translation: ABD08027.1.
RefSeqiWP_011442211.1. NC_007778.1.
YP_486938.1. NC_007778.1.

Genome annotation databases

EnsemblBacteriaiABD08027; ABD08027; RPB_3331.
GeneIDi3911133.
KEGGirpb:RPB_3331.
PATRICi23301417. VBIRhoPal125544_3481.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000250 Genomic DNA. Translation: ABD08027.1 .
RefSeqi WP_011442211.1. NC_007778.1.
YP_486938.1. NC_007778.1.

3D structure databases

ProteinModelPortali Q2IUT3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 316058.RPB_3331.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD08027 ; ABD08027 ; RPB_3331 .
GeneIDi 3911133.
KEGGi rpb:RPB_3331.
PATRICi 23301417. VBIRhoPal125544_3481.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci RPAL316058:GHF1-3375-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HaA2.

Entry informationi

Entry nameiBIOB_RHOP2
AccessioniPrimary (citable) accession number: Q2IUT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 7, 2006
Last modified: September 3, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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