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Q2IUT3 (BIOB_RHOP2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:RPB_3331
OrganismRhodopseudomonas palustris (strain HaA2) [Complete proteome] [HAMAP]
Taxonomic identifier316058 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353Biotin synthase HAMAP-Rule MF_01694
PRO_0000381580

Sites

Metal binding661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1101Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1411Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2011Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2741Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2IUT3 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: B41B571481D9566F

FASTA35338,426
        10         20         30         40         50         60 
MNSIDLASLA AATPTLRHDW TREQAAAIYN LPFADLIFRA QTIHRQSFDA NEVQCNQLLN 

        70         80         90        100        110        120 
VKTGGCAEDC GYCSQSAHHD TALPASKLMD PKKVIEGAKA ARDAGATRYC MGAAWRSPKD 

       130        140        150        160        170        180 
RDMAPVIEMV KGVKALGMEA CMTLGMLTDD QAQQLADAGL DYYNHNIDTS EEFYSSVVKS 

       190        200        210        220        230        240 
RSFGDRLDTL ATVQDAGIKV CCGGILGLGE KPTDRVEMLR TLANLPQHPE SVPINMLIPI 

       250        260        270        280        290        300 
EGTPIAKTAK PVDPFEFVRT IALARIMMPK SDVRLAAGRT AMSDEMQSLC FLAGANSIFI 

       310        320        330        340        350 
GDTLLTTPNP GDSKDRMLFA RLGITPREGA PVEAHSHDHD HDHHDHHHGH SHS 

« Hide

References

[1]"Complete sequence of Rhodopseudomonas palustris HaA2."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Pelletier D.A. expand/collapse author list , Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HaA2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000250 Genomic DNA. Translation: ABD08027.1.
RefSeqYP_486938.1. NC_007778.1.

3D structure databases

ProteinModelPortalQ2IUT3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING316058.RPB_3331.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD08027; ABD08027; RPB_3331.
GeneID3911133.
KEGGrpb:RPB_3331.
PATRIC23301417. VBIRhoPal125544_3481.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycRPAL316058:GHF1-3375-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_RHOP2
AccessionPrimary (citable) accession number: Q2IUT3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 7, 2006
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways