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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Rhodopseudomonas palustris (strain HaA2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:RPB_3440
OrganismiRhodopseudomonas palustris (strain HaA2)
Taxonomic identifieri316058 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000008809 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002129871 – 988Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST988

Interactioni

Protein-protein interaction databases

STRINGi316058.RPB_3440.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 474Adenylyl removaseUniRule annotationAdd BLAST474
Regioni480 – 988Adenylyl transferaseUniRule annotationAdd BLAST509

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256492.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiIPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2IUH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSAIDADI SPASLAARFA AGPQLHAPDE AEKRCADWLA DVPAELAAPI
60 70 80 90 100
RAVADRCPNL RIALQSIAEA SPYLFDLIRA DPARLLRLLR SEPETGFRAL
110 120 130 140 150
LDATAAAVAT ASDEAEVMAV LRRMKAEAAL SIALCDIGGL WPVLQVTQAL
160 170 180 190 200
TDLAVQSVQM TLRFLLRQEA ARGRIHPPNP DAPEERSGLI VLAMGKMGAG
210 220 230 240 250
ELNYSSDIDL IVFYELDAPT LAPDIEPQPF FVRVTQGLSR ILQQRRHDGY
260 270 280 290 300
VFRVDLRLRP DPASTPVALS TASALNYYER EGRTWERAAM IKARACAGDV
310 320 330 340 350
VAGEALLSEI APFVWRKHLD FAALSDVHDM KRQMQTFRGQ TEVAVEGHNV
360 370 380 390 400
KVGRGGIREI EFFAQTQQLI AGGRHPELRV RPTLAALDIL ANRDWITFQA
410 420 430 440 450
RDELSAAYQF LRRVEHRIQM IADEQTHTLP DSIEAVERFS RFFGYASREA
460 470 480 490 500
FARDLLAHLD CVQGHYSKLF EGDPTGTAKL PIDYAGGAED TVLLDHLRAL
510 520 530 540 550
GYKKPLMVAT TLQQWMAGGY RILKVEATQR AFNDFVPALI EELARAEEPD
560 570 580 590 600
NAVNAFDRLL QALHRGGRLI SLLSQNRDLL TLIALVLGAA PRLGDMLARQ
610 620 630 640 650
PQIMDGLIDP RFFGAMPDRT ELSARLAATL SDAGSYEEFL DRLRLFGQES
660 670 680 690 700
LFLIGTRILS GTVSTQQASI AFADVAEGIV GTVHDLVSKQ FVSQYGRIKD
710 720 730 740 750
QETAILAMGK LGSREMTAAS DLDLILIYDF DHEQPDSDGE RSLHGAQYFA
760 770 780 790 800
RFTQRLISAF TTRTNYGVLY DVDMRLRPSG RAGPLASRLD SFAEYQEVEA
810 820 830 840 850
WTWEHLALTR ARVISASPEF RARIEQVIRA VLTRPRDAAI ANDVAEMRAA
860 870 880 890 900
IAQEKGEGDV WDLKYAAGGM VDIDFIAQYL QLVHAATMPD ILAVGTLSAI
910 920 930 940 950
DNAARLGVLA QPDADVLRPA ARLYHDLTQI LRLCVSDKFK PETAGDDLLR
960 970 980
VLVRAGDAPD FSSLQARVKE TQAEVRAIFN RLIGGNGE
Length:988
Mass (Da):108,893
Last modified:March 7, 2006 - v1
Checksum:iF5CBB62206755DDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000250 Genomic DNA. Translation: ABD08136.1.
RefSeqiWP_011442320.1. NC_007778.1.

Genome annotation databases

EnsemblBacteriaiABD08136; ABD08136; RPB_3440.
KEGGirpb:RPB_3440.
PATRICi23301653. VBIRhoPal125544_3599.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000250 Genomic DNA. Translation: ABD08136.1.
RefSeqiWP_011442320.1. NC_007778.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316058.RPB_3440.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD08136; ABD08136; RPB_3440.
KEGGirpb:RPB_3440.
PATRICi23301653. VBIRhoPal125544_3599.

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256492.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiIPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_RHOP2
AccessioniPrimary (citable) accession number: Q2IUH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 7, 2006
Last modified: January 18, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.